Article

Identification of alpha-actinin 4 and 67 kDa laminin receptor as stage-specific markers in esophageal cancer via proteomic approaches.

Department of Clinical Oncology, University of Hong Kong, Pokfulam, Hong Kong, China.
Cancer (impact factor: 4.77). 01/2008; 110(12):2672-81. DOI:10.1002/cncr.23110 pp.2672-81
Source: PubMed

ABSTRACT Esophageal squamous cell carcinoma (ESCC) is one of the most common malignancies in the world with a very poor prognosis. The majority of ESCC patients present with advanced metastatic disease upon diagnosis. Therefore, it is important to understand the molecular mechanism in the tumor invasion process and to find new biomarkers for early diagnosis and prognostic evaluation.
Differentially expressed proteins among different stages of primary ESCCs and their matched surrounding normal tissues were compared by proteomics-based technology. The correlations between interesting proteins and clinical features of ESCC were further investigated by using ESCC tissue microarray (TMA) by immunohistochemical staining.
Compared with normal tissues, a total of 18 differentially expressed proteins were identified in ESCC in this study. Among them, expression levels of alpha-actinin 4 (ACTN4) and 67 kDa laminin receptor (67LR) were progressively increased from stage I to III. Clinicopathological correlation using TMA revealed that overexpression of ACTN4 was significantly associated with advanced tumor stage (P = .026) and lymph node metastasis (P = .049), whereas overexpression of 67LR was significantly correlated with advanced tumor stage (P = .019) but not lymph node metastasis.
These findings suggested that overexpression of ACTN4 and 67 LR is associated with ESCC progression and that these biomarkers may potentially be useful to prognostic evaluation, molecular biological classification, and therapeutic targeting.

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Keywords

67 kDa laminin receptor
 
alpha-actinin 4
 
Clinicopathological correlation
 
common malignancies
 
different stages
 
ESCC patients present
 
ESCC tissue microarray
 
Esophageal squamous cell carcinoma
 
immunohistochemical staining
 
lymph node metastasis
 
molecular biological classification
 
molecular mechanism
 
new biomarkers
 
normal tissues
 
poor prognosis
 
primary ESCCs
 
prognostic evaluation
 
proteomics-based technology
 
tumor invasion process
 
tumor stage