Mrg15 null and heterozygous mouse embryonic fibroblasts exhibit DNA-repair defects post exposure to gamma ionizing radiation

Department of Cellular and Structural Biology, University of Texas Health Science Center at San Antonio, 15535 Lambda Drive, STCBM #3.100, San Antonio, TX 78245, USA.
FEBS Letters (Impact Factor: 3.34). 12/2007; 581(27):5275-81. DOI: 10.1016/j.febslet.2007.10.017
Source: PubMed

ABSTRACT MORF4-related gene on chromosome 15 (MRG15) is a core component of the NuA4/Tip60 histone acetyltransferase complex that modifies chromatin structure. We here demonstrate that Mrg15 null and heterozygous mouse embryonic fibroblasts exhibit an impaired DNA-damage response post gamma irradiation, when compared to wild-type cells. Defects in DNA-repair and cell growth, and delayed recruitment of repair proteins to sites of damage were observed. Formation of phosphorylated H2AX and 53BP1 foci was delayed in Mrg15 mutant versus wild-type cells following irradiation. These data implicate a novel role for MRG15 in DNA-damage repair in mammalian cells.

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Available from: Kaoru Tominaga, Jun 26, 2015