Immunohistochemistry and Electron Microscopy of Retrocorneal Scrolls in Syphilitic Interstitial Keratitis
Department of Ophthalmology, Tokyo Dental College Ichikawa General Hospital, Chiba, Japan. Current Eye Research
(Impact Factor: 1.64).
11/2007; 32(10):863-70. DOI: 10.1080/02713680701624705
To describe the immunohistochemical and electron microscopic characteristics of retrocorneal scrolls in syphilitic interstitial keratitis.
Five eyes of five patients with congenital syphilitic interstitial keratitis who underwent keratoplasty for corneal opacities and corneal edema were studied. The corneal buttons were processed for histologic examination with hematoxylin and eosin staining and underwent immunohistochemistry stainings for collagen types I, III, IV, V, VI, VIII, fibronectin, laminin, and decorin. The corneal buttons were also processed for transmission electron microscopy and immunoelectron microscopy.
Light microscopy revealed that the retrocorneal scrolls had a multilayered, amorphous, acellular matrix. All scrolls were lined with attenuated corneal endothelial cells. The Descemet membranes in all specimens had areas of irregular thickening with attenuated endothelium. Immunohistochemical assessment of the scrolls showed positive staining for collagens I, III, IV, VI, VIII, fibronectin, laminin, and decorin but not for alpha -SMA. Immunoelectron microscopy confirmed these findings. Transmission electron microscopy showed multilaminar disorganized structures in scrolls composed of long- and short-fiber collagens.
We confirmed the presence of collagens I, III, IV, VI, VIII and proteoglycans in the retrocorneal scrolls lined with attenuated endothelium. Our findings may provide further insight into the pathogenesis of keratopathy in syphilitic interstitial keratitis.
Available from: PubMed Central
- "However, RCMs also expressed several corneal endothelium marker including VEGF and VEGFR1. This partial expression pattern of corneal endothelial markers suggests the possible involvement of both of endothelial mesenchymal transition in RCM pathogenesis as previously reported (1, 14) and progenitor cell recruitment from other sites as a new concept of pathogenesis of RCM. This finding is supportive of the argument that RCMs do not originate from corneal endothelia. "
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ABSTRACT: The purpose of this study was to elucidate the origin and cellular composition of retrocorneal membranes (RCMs) associated with chemical burns using immunohistochemical staining for primitive cell markers. Six cases of RCMs were collected during penetrating keratoplasty. We examined RCMs with hematoxylin and eosin (H&E), periodic acid-Schiff (PAS) staining and immunohistochemical analysis using monoclonal antibodies against hematopoietic stem cells (CD34, CD133, c-kit), mesenchymal stem cells (beta-1-integrin, TGF-β, vimentin, hSTRO-1), fibroblasts (FGF-β, α-smooth muscle actin), and corneal endothelial cells (type IV collagen, CD133, VEGF, VEGFR1). Histologic analysis of RCMs revealed an organized assembly of spindle-shaped cells, pigment-laden cells, and thin collagenous matrix structures. RCMs were positive for markers of mesenchymal stem cells including beta-1-integrin, TGF-β, vimentin, and hSTRO-1. Fibroblast markers were also positive, including FGF-β and α-smooth muscle actin (SMA). In contrast, immunohistochemical staining was negative for hematopoietic stem cell markers including CD34, CD133 and c-kit as well as corneal endothelial cell markers such as type IV collagen, CD133 except VEGF and VEGFR1. Pigment-laden cells did not stain with any antibodies. The results of this study suggest that RCMs consist of a thin collagen matrix and fibroblast-like cells and may be a possible neogenetic structure produced from a lineage of bone marrow-derived mesenchymal stem cells.
Journal of Korean Medical Science 06/2014; 29(6):846-51. DOI:10.3346/jkms.2014.29.6.846 · 1.27 Impact Factor
Available from: Christopher J Murphy
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ABSTRACT: The morphology of a duplication phenomenon of the canine Descemet's membrane (DM) is described in relation to signalment, history, and ocular disease status. Sixty-six canine eyes from the Comparative Ocular Pathology Laboratory of Wisconsin archives between 2000 and 2007 were examined. All cases were stained with hematoxylin and eosin and Alcian blue periodic acid-Schiff (PAS), while 14 cases were additionally stained with Masson's trichrome, picrosirius red, cytokeratin AE1/AE3 (CK), vimentin, and alpha-smooth muscle actin (SMA). Transmission electron microscopy (TEM) examination was performed in 3 corneas and in 1 normal control eye. Alcian blue PAS staining and TEM confirmed the basement membrane nature of the abnormal secondary DM. The thickness of the first DM, referred to as the corneal layer (CL) and the second or anterior chamber layer (ACL), were nearly the same, with no significant difference seen (P = .93). In 39% (26/66) of the eyes, a fibrous, collagenous matrix component was present between the CL and ACL, which contains vimentin-positive and alpha-SMA-negative spindle cells (14/14).The corneal endothelial cells in 7/14 eyes stained weakly with CK and strongly in 2 additional eyes. The most frequent histopathologically confirmed, clinical ocular histories were chronic glaucoma in 76% (50/66) of eyes, previous intraocular surgery in 36% (24/66), lens luxation in 21% (4/66), and blunt trauma in 15% (10/66) of the cases. We speculate that activation and migration of endothelial cells, in association with trauma or lens contact, play a role in the pathogenesis of this phenomenon.
Veterinary Pathology 02/2009; 46(3):464-73. DOI:10.1354/vp.08-VP-0183-K-FL · 1.87 Impact Factor
The American Journal of dermatopathology 07/2010; 32(5):523-5. DOI:10.1097/DAD.0b013e3181c22546 · 1.39 Impact Factor
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