The severity of systemic lupus erythematosus negatively correlates with the increasing number of CD4(+)CD25(high)FoxP3(+) regulatory T cells during repeated plasmapheresis treatments of patients
ABSTRACT Systemic lupus erythematosus (SLE) is a systemic autoimmune disease characterized by increased pathologic autoantibody production. A decrease in the number of CD4+CD25(high)FoxP3+ regulatory T cells can play a key role in the loss of tolerance to self antigens. Our aim was to determine the absolute number of peripheral CD4+CD25(high)FoxP3+ T cells in 44 patients with SLE, furthermore, to measure the changes in the number of CD+CD25(high)FoxP3+ T cells in 5 patients with severe SLE treated with repeated plasmapheresis for 4-6 days in comparison to the changes in the activity of disease (SLEDAI). Percent of CD4+CD25(high)FoxP3+ T cells were measured by flow cytometry. The absolute number of peripheral CD4+CD25(high)FoxP3+ T cells was significantly decreased in the 44 patients with SLE compared to the healthy controls n = 32 (0.012 +/- 0.006 vs. 0.038 +/- 0.017 G/L, p < 0.05). In the 5 patients with severe SLE the repeated plasmapheresis treatments increased the peripheral number of CD4+CD25(high)FoxP3+ T cells. As the number of CD4+CD25(high)FoxP3+ T cells increased during the treatment, the activity of disease (the value of SLE activity index) decreased. In the peripheral blood of SLE patients not only the ratio was decreased (as it was published earlier) but also the absolute number of these regulatory T cells. The repeated plasmapheresis treatments of SLE patients induced a significant increase in the number of peripheral CD4+CD25(high)FoxP3+ T cells in parallel to the decrease in the values of SLEDAI (the activity of disease). This phenomenon is, among others, possibly due to the elimination of interpheron-alpha and lymphocytotoxic antibodies during plasmapheresis.
Conference Paper: Control of a multimodule deployable manipulator using RTX[Show abstract] [Hide abstract]
ABSTRACT: A multimodule deployable manipulator system (MDMS), which has attractive features of kinematics and kinetics, has been developed in our laboratory. The present paper concerns implementation and evaluation of a low-level real-time controller for the MDMS, using VenturCom's real-time extension (RTX) for Windows NT in the host computer. A software module is developed using C/C++ with RTX for implementation of various control methods. As a preliminary study of the effectiveness of the control system, a conventional proportional-integral-derivative (PID) control scheme is implemented for simultaneous multilink independent joint motion of the MDMS. Several experiments of circle-trajectory following are carried out using two degrees of freedom of the outer module of the robot The performance is found to be quite accurate, providing greater accuracies at lower speeds. Further improvements in performance are realized by tuning the parameters of the PID controllers.Communications, Computers and signal Processing, 2003. PACRIM. 2003 IEEE Pacific Rim Conference on; 09/2003
Conference Paper: High step-up DC-DC converter for fuel cell generation system[Show abstract] [Hide abstract]
ABSTRACT: Due to the electrochemical reaction, fuel cell has the power quality of low voltage as well as high current. However, the fuel cell stack with high output voltage is difficult to fabricate and its volume is overlarge. Besides, the output voltage of the fuel cell is varied easily with respect to the variation of loads. In order to satisfy the requirement of high-voltage demand, a high-efficiency DC-DC converter with superior voltage gain is one of the essential mechanisms in fuel cell applications. In this study, a newly-designed DC-DC converter utilized in the proton exchange membrane fuel cell (PEMFC) system is constructed on the basis of voltage-clamped and soft-switching techniques for alleviating the switching and conduction losses to further increase the conversion efficiency. The effectiveness of the proposed converter used for the PEMFC system is verified by experimental results.Industrial Electronics Society, 2004. IECON 2004. 30th Annual Conference of IEEE; 12/2004
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ABSTRACT: The aim of our study was to investigate and characterize regulatory T cells (Treg) in peripheral blood of patients with connective tissue diseases (Systemic lupus erythematosus, systemic sclerosis, Sjögren's syndrome, poly- and dermatomyositis) as compared with blood from healthy controls. Treg cells were quantified and phenotypically characterized by flow cytometry while the expression level of Foxp3 mRNA was evaluated by real time PCR. A reduced percentage of peripheral blood Treg cells was found in patients than in controls, irrespective of the type of connective tissue disease. Treg cells, especially those expressing one of the phenotypical markers, seemed to differ not only between patients and healthy controls but also among types of diseases. Additionally, the presence of autoantibodies as well as disease activity appeared to be correlated with particular Treg cell populations, especially those expressing one of the examined phenotypical markers. Correlations with therapy suggested that glucocorticoids plus antimalarial or other immunosuppressor drugs diminished the percentage of Treg cells, especially of those with memory phenotype. These findings indicated dysregulations at the level of Treg cells and suggested an involvement of these cells in the pathology of connective tissue diseases. Moreover, our data are in agreement with the suggestion that Treg cells could be therapeutic targets for some autoimmune diseases.Autoimmunity 10/2008; 42(1):41-9. DOI:10.1080/08916930802282651 · 2.75 Impact Factor