Temporal profile of circadian clock gene expression in a transplanted suprachiasmatic nucleus and peripheral tissues

Department of Anatomy and Neurobiology, Kinki University School of Medicine, 377-2 Ohno-Higashi, Osakasayama City, Osaka 589-8511, Japan.
European Journal of Neuroscience (Impact Factor: 3.18). 12/2007; 26(10):2731-8. DOI: 10.1111/j.1460-9568.2007.05926.x
Source: PubMed


The mammalian hypothalamic suprachiasmatic nucleus (SCN) is the master oscillator that regulates the circadian rhythms of the peripheral oscillators. Previous studies have demonstrated that the transplantation of embryonic SCN tissues into SCN-lesioned arrhythmic mice restores the behavioral circadian rhythms of these animals. In our present study, we examined the clock gene expression profiles in a transplanted SCN and peripheral tissues, and also analysed the circadian rhythm of the locomotor activity in SCN-grafted mice. These experiments were undertaken to elucidate whether the transplanted SCN generates a dynamic circadian oscillation and maintains the phase relationships that can be detected in intact mice. The grafted SCN indeed showed dynamic circadian expression rhythms of clock genes such as mPeriod1 (mPer1) and mPeriod2 (mPer2). Furthermore, the phase differences between the expression rhythms of these genes in the grafted SCN and the locomotor activity rhythms of the transplanted animals were found to be very similar to those in intact animals. Moreover, in the liver, kidney and skeletal muscles of the transplanted animals, the phase angles between the circadian rhythm of the grafted SCN and that of the peripheral tissues were maintained as in intact animals. However, in the SCN-grafted animals, the amplitudes of the mPer1 and mPer2 rhythms were attenuated in the peripheral tissues. Our current findings therefore indicate that a transplanted SCN has the capacity to generate a dynamic intrinsic circadian oscillation, and can also lock the normal phase angles among the SCN, locomotor activity and peripheral oscillators in a similar manner as in intact control animals.

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    • "). In line with this idea, a recent study demonstrated that peripheral clock gene oscillations and their phase relation between organs were restored by SCN grafts despite “aberrant” locomotor activity (i.e., compared to intact animal) and disturbed endocrine rhythm [66], [67]. Interestingly, only a limited number of studies provided a real insight into the clock gene function, e.g., cell division [68], [69], chromatin remodeling [70] and haem metabolism [71]. "
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