Fatty acids, antioxidants, and oxidative stress in pre-eclampsia
ABSTRACT To investigate whether free radical-mediated membrane lipid peroxidation may be implicated in the pathogenesis of pre-eclampsia.
A prospective study using a sample of 55 healthy pregnant women and 60 pre-eclamptic women recruited at Bharati Medical Hospital, Pune, India. Maternal and cord samples were examined for (red blood cells and plasma) fatty acid profiles, antioxidants, and oxidative stress levels. Mean values were compared between case and control groups using the t test and Wilcoxon rank test.
Pre-eclamptic women showed reduced total omega-3 fatty acids (P<0.05), increased omega-6:omega-3 ratio (P<0.05), higher oxidative stress (P<0.05), and lower antioxidant (P<0.05) levels. Similar trends were also observed in cord samples.
Reduced antioxidants and increased oxidative stress leading to impaired essential polyunsaturated fatty acid levels may be a key factor in the development of pre-eclampsia.
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ABSTRACT: Preeclampsia (PE) is one of the main causes of maternal and fetal mortality and morbidity. PE is associated with an inflammatory state and with oxidative stress, in maternal circulation. Our aim was to evaluate and compare the levels of oxidative stress and inflammatory markers in maternal and umbilical cord blood (UCB), in normal and PE pregnancies. We measured acute-phase proteins (CRP and α1-antitrypsin), proinflammatory cytokines (IL-6 and TNF-α), leukocyte activation (elastase, lactoferrin, sL-selectin, sVCAM, sPECAM), total antioxidant status (TAS), thiobarbituric acid reactive substances (TBARS), and uric acid levels. We studied 42 healthy pregnant women, 46 PE women, and their neonates. The concentrations of IL-6, TNF-α, α1-antitrypsin, CRP, sVCAM, uric acid, and TBARS were significantly higher, and sL-selectin was significantly lower in PE pregnant women as compared with normotensive pregnant women. In newborns uric acid, α1-antitrypsin, and CRP values were significantly higher in PE; leukocyte count, sL-selectin, lactoferrin, and the ratio elastase/α1-antitrypsin were significantly lower. Our data suggest that PE pregnancy is associated with an enhanced maternal inflammatory condition, which is reflected in fetal circulation. This enhanced inflammatory state seems to be related to endothelial dysfunction and increased cytokine synthesis, rather than with neutrophil activation.Journal of pregnancy 05/2012; 2012:684384. DOI:10.1155/2012/684384
- Oxidative Stress in Vertebrates and Invertebrates: Molecular Aspects of Cell Signaling, 11/2011: pages 209 - 234; , ISBN: 9781118148143
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ABSTRACT: The present study evaluated the hypothesis that the effects of hypoxia on sheep pregnancies at high altitude (HA) are mediated by oxidative stress and that antioxidant vitamins may prevent these effects. Both HA native and newcomer ewes were maintained at an altitude of 3,589 m during mating and pregnancy. Control low altitude (LA) native ewes were maintained at sea level. Half of each group received daily oral supplements of vitamins C (500 mg) and E (350 IU) during mating and gestation. Near term, maternal plasma vitamin levels and oxidative stress biomarkers were measured. At delivery, lambs were weighed and measured, and placentas were recovered for macroscopic and microscopic evaluation. Vitamin concentrations in supplemented ewes were two- or threefold greater than in non-supplemented ewes. Plasma carbonyls and malondialdehyde in non-supplemented ewes were consistent with a state of oxidative stress, which was prevented by vitamin supplementation. Vitamin supplementation increased lamb birthweight and cotyledon number in both HA native and newcomer ewes, although placental weight and cotyledon surface were diminished. Placentas from vitamin-supplemented HA ewes were similar to those from ewes at sea level, making these placental traits (weight, number and diameter of cotyledons) similar to those from ewes at sea level. Vitamin supplementation had no effect on LA pregnancies. In conclusion, supplementation with vitamins C and E during pregnancy at HA prevents oxidative stress, improving pregnancy outcomes.Reproduction Fertility and Development 01/2011; 23(2):285-96. DOI:10.1071/RD10016 · 2.58 Impact Factor