Nasal Carriage of Methicillin-Resistant Staphylococcus aureus in Household Contacts of Children With Community-Acquired Diseases in Taiwan

Division of Pediatric Infectious Diseases, Chang Gung Children's Hospital, Taoyuan, Taiwan.
The Pediatric Infectious Disease Journal (Impact Factor: 2.72). 12/2007; 26(11):1066-8. DOI: 10.1097/INF.0b013e31813429e8
Source: PubMed


Nasal carriage of methicillin-resistant Staphylococcus aureus was detected in 18 (32%) of 57 children with community-acquired methicillin-resistant S. aureus infection and 30 (25%) of 121 household contacts. By genotyping comparison, 94% and 64% of the colonization isolates from the patients and the contacts, respectively, were indistinguishable from the clinical isolates.


Available from: Yhu-Chering Huang, Jun 09, 2015
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    • "But the rate was lower than that for healthy children, ranging from 6.2% to 9.5% between 2005 and 2008 [26]. But, for contacts, previous reports from Taiwan indicated that the nasal MRSA carriage rate was found to be 13.6% for 66 contacts with a severe case of CA-MRSA infection [20], and 25%for 121 household contacts [27]. The issue whether contact with children or patients with MRSA infection is associated with a higher MRSA carriage rate for pediatricians needs further investigations. "
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    ABSTRACT: Health care workers (HCWs) are at the interface between hospitals and communities. The survey for methicillin-resistant Staphylococcus aureus (MRSA) carriage among HCWs has mostly been conducted to investigate outbreaks or endemics. Community-associated MRSA are prevalent among children in Taiwan. We conducted this study to better understand the carriage rate of MRSA among pediatricians in non-outbreak situations in Taiwan,. A total of 220 pediatricians from Taiwan who attended the annual meeting of Taiwan Pediatric Association in April, 2010 were recruited to participate in this study and were sampled from the nares for the detection of MRSA by polymerase chain reaction (PCR) and further by culture. The following molecular analyses were performed, including pulsed-field gel electrophoresis (PFGE), multilocus sequence typing (MLST), typing of staphylococcal cassette chromosome mec (SCCmec) and the presence of Panton-Valentine leukocidin (PVL) genes. MRSA was detected from 15 attendees (6.8%) by PCR. MRSA-colonized attendees had a significantly lower rate (0.041) of working in the medical center, while borderline significantly higher rate of working in the Regional Hospital (p=0.056), than those without MRSA colonization. From those 15 samples, 12 MRSA isolates were identified by culture and molecularly characterized. Three PFGE patterns, two sequence types (ST 59, ST 508), and two SCCmec types (IV and VT) were identified, respectively. Five isolates, including three carrying SCCmec types VT, were PVL-positive. All 12 isolates were susceptible to vancomycin, teicoplanin, linezolid, fusidic acid, trimethoprim/sulfamethoxazole, and doxycyclin, and resistant to penicillin. Around seven percent of pediatricians in Taiwan harbored CA-MRSA in their nares.
    PLoS ONE 11/2013; 8(11):e82472. DOI:10.1371/journal.pone.0082472 · 3.23 Impact Factor
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    • "Studies have demonstrated the prevalence of different genotypes among contacts of patients with CA-MRSA [21,31]. In our study, direct spread between family contacts cannot completely explain the increased colonization of MRSA as different strain lineages were isolated among some of our study group families. "
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    ABSTRACT: To measure Methicillin-resistant Staphylococcus aureus (MRSA) nasal colonization prevalence in household contacts of children with current community associated (CA)-MRSA infections (study group) in comparison with a group of household contacts of children without suspected Staphylococcus aureus infection (a control group). This is a cross sectional study. Cultures of the anterior nares were taken. Relatedness of isolated strains was tested using pulse field gel electrophoresis (PFGE). The prevalence of MRSA colonization in the study group was significantly higher than in the control group (18/77 (23%) vs 3/77 (3.9%); p ≤ 0.001). The prevalence of SA colonization was 28/77 (36%) in the study group and 16/77 (21%) in the control group (p = 0.032). The prevalence of SA nasal colonization among patients was 6/24 (25%); one with methicillin-susceptible S. aureus (MSSA) and 5 with MRSA. In the study (patient) group, 14/24 (58%) families had at least one household member who was colonized with MRSA compared to 2/29 (6.9%) in the control group (p = 0.001). Of 69 total isolates tested by PFGE, 40 (58%) were related to USA300. Panton-Valetine leukocidin (PVL) genes were detected in 30/52 (58%) tested isolates. Among the families with ≥1 contact colonized with MRSA, similar PFGE profiles were found between the index patient and a contact in 10/14 families. Prevalence of asymptomatic nasal carriage of MRSA is higher among household contacts of patients with CA-MRSA disease than control group. Decolonizing such carriers may help prevent recurrent CA-MRSA infections.
    BMC Infectious Diseases 02/2012; 12(1):45. DOI:10.1186/1471-2334-12-45 · 2.61 Impact Factor
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    • "Central 2017 125 (6.2) Southern 2023 156 (7.7) Contacts School children 10–12 years 2001 Northern 63 8 (13) 100 [50] Household Any 2004–2005 Northern 121 30 (25) 93 [58] Household Any 2007 Northern 242 64 (26) N/A [56] Healthcare workers Ordinary ward Adults 2001 Northern 76 5 (6.6) 100 [50] Ordinary ward Adults 2001 Southern 139 7 (5.0) N/A [51] NICU Adults 2003–2004 Northern 384 30 (7.8) 83 [6] "
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    ABSTRACT: Meticillin-resistant Staphylococcus aureus (MRSA) has been increasingly identified as the major cause of community-associated (CA) infections in previously healthy hosts since the late 1990s. CA-MRSA strains were recognised as a novel pathogen that is genetically different from healthcare-associated MRSA, and five major epidemic clones have been identified worldwide. In Taiwan, a significantly increasing rate of MRSA carriage and infection amongst healthy subjects was observed in the past decade. Up to 9.5% of healthy Taiwanese children carried MRSA in the nares and >50% of paediatric CA S. aureus infections were MRSA. The adult population was also affected, but this was relatively limited. The majority of CA-MRSA isolates in Taiwan belonged to the sequence type (ST) 59 lineage, defined by multilocus sequence typing, and were multiresistant to non-β-lactams. The clone of ST59 lineage can be further classified into at least two major clones by pulsed-field gel electrophoresis (PFGE) typing, staphylococcal chromosomal cassette mec (SCCmec) elements and Panton-Valentine leukocidin (PVL) genes. The clone characterised as ST59/PFGE type C/SCCmec IV/PVL-negative was prevalent amongst the colonising isolates, whereas ST59/PFGE type D/SCCmec V(T)/PVL-positive was prevalent amongst the clinical isolates. Evidence suggested that the ST59 CA-MRSA clone was not only circulating in Taiwan but also in other areas of the world. In this article, the current status of CA-MRSA in Taiwan was extensively reviewed. The information provided here is not only important for local public health but can also enhance a general understanding of the successful epidemic clones of CA-MRSA worldwide.
    International journal of antimicrobial agents 03/2011; 38(1):2-8. DOI:10.1016/j.ijantimicag.2011.01.011 · 4.30 Impact Factor
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