Yeast cell-surface expression of chitosanase from Paenibacillus fukuinensis

Department of Applied Chemistry and Biotechnology, Graduate School of Engineering, University of Fukui, Japan.
Bioscience Biotechnology and Biochemistry (Impact Factor: 1.21). 12/2007; 71(11):2845-7. DOI: 10.1271/bbb.70315
Source: PubMed

ABSTRACT To produce chitoorigosaccharides using chitosan, we attempted to construct Paenibacillus fukuinensis chitosanase-displaying yeast cells as a whole-cell biocatalyst through yeast cell-surface engineering. The localization of the chitosanase on the yeast cell surface was confirmed by immunofluorescence labeling of cells. The chitosanase activity of the constructed yeast was investigated by halo assay and the dinitrosalicylic acid method.

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