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Byrne JA, Pedersen DA, Clepper LL, et al. Producing primate embryonic stem cells by somatic cell nuclear transfer. Nature 2007;450:497-502

Oregon National Primate Research Center, Oregon Health & Science University, 505 N.W. 185th Avenue, Beaverton, Oregon 97006, USA.
Nature (Impact Factor: 42.35). 12/2007; 450(7169):497-502. DOI: 10.1038/nature06357
Source: PubMed

ABSTRACT Derivation of embryonic stem (ES) cells genetically identical to a patient by somatic cell nuclear transfer (SCNT) holds the potential to cure or alleviate the symptoms of many degenerative diseases while circumventing concerns regarding rejection by the host immune system. However, the concept has only been achieved in the mouse, whereas inefficient reprogramming and poor embryonic development characterizes the results obtained in primates. Here, we used a modified SCNT approach to produce rhesus macaque blastocysts from adult skin fibroblasts, and successfully isolated two ES cell lines from these embryos. DNA analysis confirmed that nuclear DNA was identical to donor somatic cells and that mitochondrial DNA originated from oocytes. Both cell lines exhibited normal ES cell morphology, expressed key stem-cell markers, were transcriptionally similar to control ES cells and differentiated into multiple cell types in vitro and in vivo. Our results represent successful nuclear reprogramming of adult somatic cells into pluripotent ES cells and demonstrate proof-of-concept for therapeutic cloning in primates.

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    • "In rhesus monkey SCNT-ESC lines have been obtained (Byrne et al., 2007), but the live birth of cloned animals has not yet been reported. Compared with normal embryos, the ICM cells of cloned embryos maintain a high level of DNA methylation and this may disturb normal embryo development after SCNT (Yang et al., 2007). "
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    • "Quantitative Analysis The most common comparison at the gene expression level is done by looking at the transcriptome of the cells and by comparing gene expression profiles. In niche (Byrne et al., 2007). Particularly, it is the existence of a functional hierarchy among signaling molecules, which contribute to either the initiation of large-scale phenotypic change or the maintenance of the current state. "
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    • "The general value of polarized light microscopy for the biomedical sciences lies in the label-free imaging of structural parameters that can be followed real-time while cells and tissues are functioning under physiological conditions. One example of this is the use of the LC-PolScope technique to image the meiotic spindle in egg cells undergoing procedures for in-vitro fertilization and for cloning (Byrne et al. 2007; Keefe et al. 2003; Liu et al. 2000). In this article we review the use of the LC-PolScope for generating maps of birefringence measured in transparent specimens, and introduce a new use of the instrument, requiring a slight modification, to create maps of linear and circular diattenuation. "
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