Article

[Effect of cold preservation and reperfusion injury on early-stage bile salt secretion after liver transplantation in rat model].

Institute of Hepatobiliary Surgery, Southwest Hospital, Third Military Medical University, Southwest Hospital of Hepatobiliary Surgery, Chongqing 400038, China.
Zhonghua wai ke za zhi [Chinese journal of surgery] 09/2007; 45(15):1027-30. pp.1027-30
Source: PubMed

ABSTRACT To explore the effect of cold preservation and reperfusion injury (CPRI) on the bile salt spectrum in rat orthotopic liver transplantation (OLT) model.
A special analysis method was established to investigate the bile salts in rat by reverse phase high performance liquid chromatography (RP-HPLC). Rats were randomly divided into 3 groups: group A (control group, n = 6), group B (group with 1 h graft preservation pre-OLT, n = 6) and group C (group with 12 h graft preservation pre-OLT, n = 6). The bile samples of 0 - 14 post-transplantation days were analyzed by RP-HPLC.
Eleven kinds of bile salts were detected in rat bile. It showed that CPRI could influence the concentration of bile salts significantly in rat model after OLT, the concentration of hydrophobic bile salts (TCA and TCDCA) increased significantly in group B and C. However, the concentration of hydrophilic bile salts (TUDCA and THDCA) just increased in a short-time. The hydrophobicity index (HI) wasn't significantly changed during the first 4 post-transplant days. Thus the HI of bile salts elevated gradually from the 5th day and reached the peak at the 10th day after OLT.
The increase of the proportion of hydrophobic bile salts may be one of the major factors leading to the increase of bile toxicity after OLT.

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Keywords

1 h graft preservation pre-OLT
 
12 h graft preservation pre-OLT
 
14 post-transplantation days
 
3 groups
 
5th day
 
bile salt spectrum
 
bile salts
 
bile samples
 
control group
 
first 4 post-transplant days
 
group B
 
hydrophilic bile salts
 
hydrophobic bile salts
 
hydrophobicity index
 
major factors
 
rat bile
 
rat model
 
rat orthotopic liver transplantation
 
reverse phase
 
special analysis method