Enhanced tolerance of transgenic potato plants overexpressing nucleoside diphosphate kinase 2 against multiple environmental stresses. Transgenic Res

Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, 52 Eoeun-dong, Yuseong-gu, Daejeon, Korea.
Transgenic Research (Impact Factor: 2.32). 09/2008; 17(4):705-15. DOI: 10.1007/s11248-007-9155-2
Source: PubMed


In plants, nucleoside diphosphate kinase 2 (NDPK2) is known to regulate the expression of antioxidant genes. In this study, we developed transgenic potato plants (Solanum tuberosum L. cv. Atlantic) expressing Arabidopsis NDPK2 (AtNDPK2) gene in cytosols under the control of an oxidative stress-inducible SWPA2 promoter (referred to as SN plants) or enhanced CaMV 35S promoter (EN plants) and evaluated their tolerance to various environmental stress, including methyl viologen (MV)-mediated oxidative stress, high temperature, and salt stress. When 250 muM MV was sprayed to whole plants, plants expressing NDPK2 showed significantly an enhanced tolerance compared to non-transgenic (NT) plants. SN plants and EN plants showed 51% and 32% less visible damage than NT plants, respectively. Transcript level of AtNDPK2 gene and NDPK2 activity in SN plants following MV treatment well reflected the plant phenotype. Ascorbate peroxidase (APX) activity was also increased in MV-treated SN plants. In addition, SN plants showed enhanced tolerance to high temperature at 42 degrees C. The photosynthetic activity of SN plants after treatment of high temperature was decreased by about 10% compared to the plants grown at 25 degrees C, whereas that of NT plants declined by 30%. When treated with 80 mM NaCl onto the plantlets, both SN plants and EN plants also showed a significant reduced damage in root growth. These results indicate that overexpression of NDPK2 under the stress-inducible SWPA2 promoter might efficiently regulate the oxidative stress derived from various environmental stresses.

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Available from: Suk-Yoon Kwon, Dec 18, 2013
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    • "The expression of the GUS reporter gene driven by the SWPA2 promoter in transgenic tobacco plants is strongly induced in response to environmental stresses including H 2 O 2 , wounding and UV treatment (Kim et al., 2003). In addition, transgenic plants expressing various stress-tolerance genes under the control of the SWPA2 promoter exhibit increased tolerance to methyl viologen (MV)-induced oxidative stress as well as salt, drought, and temperature stresses (Tang et al., 2008; Kim et al., 2009, 2010, 2011). These results suggest that the SWPA2 promoter will be useful for the development of transgenic plants with enhanced tolerance to environmental stresses. "
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    • "Salt stress triggers oxidative stress in plant tissues; salt stress could reduce gas exchange and limit CO2 supply to the leaf [5], which causes an over-reduction of the photosynthetic electron transport chain [48] and concomitant production of ROS such as singlet oxygen (1O2), O2−, H2O2, and OH- [5], [49]. Thus, excessive ROS induced by salt stress must be removed in time to avoid plants suffered serious oxidative damage although they may also signal the induction of protection mechanisms [6], [50], [51]. In this study, we gradually increase the salt concentration to acclimate the plants to salt stress for cotton moderate tolerance [52], and our transgenic and control plants could flower and set seed. "
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    PLoS ONE 01/2013; 8(1):e54002. DOI:10.1371/journal.pone.0054002 · 3.23 Impact Factor
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    • "Nucleoside diphosphate kinase (NDPK) is a ubiquitous enzyme functioning in intracellular distribution of terminal phosphate bond energy among the various nucleotides used in synthesis pathways and regulatory functions in cells [43,44]. Plant NDPKs are also involved in signal transduction, differentiation and development [45-47]. Our results showed that NDPK was gradually down-regulated during grain development in both wheat cultivars (Figure 9), and therefore might play an important role in signal regulation for grain development. "
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