Article

Diagnostic multiplex PCR assay for the identification of the Liverpool, Midlands 1 and Manchester CF epidemic strains of Pseudomonas aeruginosa

Division of Medical Microbiology, University of Liverpool, Daulby Street, Liverpool L69 3GA, United Kingdom.
Journal of Cystic Fibrosis (Impact Factor: 3.82). 06/2008; 7(3):258-61. DOI: 10.1016/j.jcf.2007.09.002
Source: PubMed

ABSTRACT Individual PCR amplification tests have been developed for three UK CF epidemic strains, the Liverpool epidemic strain (LES), Midlands 1 and the Manchester epidemic strain (MES). We report a simple diagnostic multiplex PCR test that can be used to screen for all three of these strains. To evaluate the test, we screened collections of LES, MES and Midlands 1 isolates, along with various CF and non-CF non-epidemic Pseudomonas aeruginosa strains. The test was 100% sensitive and 100% specific in the identification of these UK CF epidemic strains.

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    International journal of antimicrobial agents 07/2013; 42(4). DOI:10.1016/j.ijantimicag.2013.05.010 · 4.26 Impact Factor
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    • "2008–2009: In March 2008, by combining the specific primers and modifying the product mix we identified LES, Manchester and Midlands1 strains from one test (combined multiplex PCR).26 Primer F9 added to the PCR mix increased the specificity of the test to identify LES. "
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    01/2013; 4(1):1. DOI:10.1258/shorts.2012.012018
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    • "All patients submit microbiological samples at every clinic visit/inpatient stay: chronic infection with Psa is defined by at least three positive sputum samples within a 6-month period.10 Our unit has pioneered Psa genotyping methodology in CF, and we regularly genotype the Psa isolates from our patients to aid cross-infection control measures within the clinic: those without known LES infection undergo genotyping using PCR every 3 months to identify unique and other transmissible Psa strains,11 while those infected with LES undergo a genotypic check (using PCR and primers PS21 & F9)12 on a yearly basis. Using this system, we are aware of the Psa genotypes infecting our CF patients at all times. "
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