Induction Of Pluripotent Stem Cells From Adult Human Fibroblasts By Defined Factors

Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606-8507, Japan.
Cell (Impact Factor: 32.24). 12/2007; 131(5):861-72. DOI: 10.1016/j.cell.2007.11.019
Source: PubMed


Successful reprogramming of differentiated human somatic cells into a pluripotent state would allow creation of patient- and disease-specific stem cells. We previously reported generation of induced pluripotent stem (iPS) cells, capable of germline transmission, from mouse somatic cells by transduction of four defined transcription factors. Here, we demonstrate the generation of iPS cells from adult human dermal fibroblasts with the same four factors: Oct3/4, Sox2, Klf4, and c-Myc. Human iPS cells were similar to human embryonic stem (ES) cells in morphology, proliferation, surface antigens, gene expression, epigenetic status of pluripotent cell-specific genes, and telomerase activity. Furthermore, these cells could differentiate into cell types of the three germ layers in vitro and in teratomas. These findings demonstrate that iPS cells can be generated from adult human fibroblasts.

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Available from: Kiichiro Tomoda, Jul 28, 2014
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    • "Recent advancement in stem cell research makes it possible to generate and propagate induced pluripotent stem cells (iPSCs) from skin biopsy or urine samples (containing exfoliated renal epithelial cells; Takahashi et al., 2007; Yu et al., 2007; Zhou et al., 2012). DNA can then be extracted from iPSC cultures for use in genetic analyses. "
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    Methods in enzymology 12/2015; 552:309-24. DOI:10.1016/bs.mie.2014.10.046 · 2.09 Impact Factor
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    • "The detection and breeding of MHC homozygous primate species are considered necessary for the development of effective vaccine and immunosuppressive drug protocols, evaluation , and validation of organ and regenerated cells originating from induced pluripotent stem (iPS) cells and/or embryonic stem (ES) cells in transplantation medicine (Klimanskaya et al. 2006; Takahashi et al. 2007). In this regard, the cynomolgus macaques from Mauritius and the Philippines are considered the most suitable populations for use in biomedical research because they are thought to be genetically less diverse than those from other geographic locations (Kawamoto et al. 2008; Tosi and Coke 2007; Wiseman et al. 2007). "
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    • "Stem cells generated by this new technology were termed induced pluripotent stem cells (iPSCs). Since their discovery, there has been substantial interest in iPSCs, as this technology facilitates the generation of adult human pluripotent stem cells without the need for human embryos, thereby bypassing a number of the ethical and legal concerns that have hindered embryonic stem cell research to date (Takahashi and Yamanaka 2006; Maherali et al. 2007; Okita et al. 2007; Takahashi et al. 2007; Wernig et al. 2007). iPSCs appear to have a number of advantages over other dental-derived stem cell populations, such as periodontal ligament (PDL) and dental pulp. "
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