β-Carotene Isomer Composition of Sub- and Supercritical Carbon Dioxide Extracts. Antioxidant Activity Measurement

Sección Departamental de Ciencias de la Alimentación, Universidad Autónoma de Madrid, Spain.
Journal of Agricultural and Food Chemistry (Impact Factor: 2.91). 01/2008; 55(26):10585-90. DOI: 10.1021/jf0711789
Source: PubMed


In the present work sub- and supercritical extraction conditions using carbon dioxide were studied in order to obtain extracts with different compositions from the green microalgae Dunaliella salina. Different compositions of beta-carotene isomers were identified in the extracts by using HPLC-DAD. Also, antioxidant activity of the extracts was measured using a TEAC assay. An experimental design was applied considering two factors, extraction pressure and temperature, in a wide range of values, trying to maximize the extraction yield. Higher yields were obtained at high pressures and low temperatures, that is, at higher CO2 densities. Attempts were made to correlate the antioxidant activity of the extracts with their chemical composition by means of principal component analysis. A certain relationship was found between their antioxidant activity and the isomeric composition of beta-carotenes. As a result, an original equation is proposed to predict the antioxidant activity of extracts from D. salina in terms of the ratio 9-cis-beta-carotene/all-trans-beta-carotene, the concentration of alpha-carotene, and, especially, the concentration of 9-cis-beta-carotene.

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    • " results indicated that the amount of all - trans form increased with the increase in pressure , from 31% of the total lycopene at 0 . 55 g / mL to 88% of the total lycopene at 0 . 90 g / mL ( Fig . 15 ) . The difference in the extraction of the cis or all - trans form is attributed to the difference in solubility in SC - CO 2 of these compounds . Jaime et al . ( 2007 ) evaluated the extraction of β - carotene isomers from Dunaliella salina ."
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