Review of factors essential for blastocyst implantation for their modulating effects on the maternal immune system

Reproductive Sciences Branch, Center for Population Research, National Institute of Child Health and Human Development, NIH, DHHS, Bethesda, MD 20892-7510, USA.
Seminars in Cell and Developmental Biology (Impact Factor: 6.27). 05/2008; 19(2):161-9. DOI: 10.1016/j.semcdb.2007.10.006
Source: PubMed


Pituitary and ovarian hormones prepare the endometrium for successful blastocyst implantation and support its process directly or indirectly through the action of growth factors, cytokines and other molecules. Many of the blastocyst implantation essential factors (BIEFs) are modulators of the maternal immune system. Since little is known as to the action of these molecules on the uterine lymphocytes, its clarification is imperative to the understanding of the process of blastocyst implantation.

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Available from: Koji Yoshinaga, Nov 10, 2015
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    • "The embryo implantation process is one of the mysteries of reproductive biology. Many aspects regarding the molecular basis of endometrial receptivity and the reciprocal interactions taking place between the blastocyst and endometrium remain unknown (Hou et al. 2004; Yoshinaga 2008). It was only in the past decade that similarities between the behaviour of placental cells and invasive cancer cells became apparent. "
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    ABSTRACT: Successful implantation relies on the interaction between a competent embryo and a receptive endometrium. The aim of the present study was to investigate genes differentially expressed in early invasive embryonic tissue versus decidual tissue in mice. Samples were obtained from the ectoplacental cone, the immediately surrounding deciduas and from deciduas from interimplantation sites. Microarray analysis showed that 817 genes were differentially expressed between extra-embryonic tissue and the surrounding decidua and that 360 genes were differentially expressed between the different deciduas, with a high representation of developmental processes. Genes differentially expressed in the maternal compartment included chemokines, lipoproteins, growth factors and transcription factors, whereas the embryonic invasive tissue expressed genes commonly observed in invasive tumour-like processes. These results provide information about genes involved in early embryonic invasion and the control exerted by the surrounding decidua. This information may be useful to find targets involved in pathologies associated with implantation failure and early pregnancy loss.
    Reproduction Fertility and Development 05/2015; DOI:10.1071/RD14166 · 2.40 Impact Factor
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    • "Once materno-zygotic contact is established, the trophectoderm cells invade into the luminal epithelium [3]. Numerous factors, such as hormones, growth factors, cytokines, vasoactive agents, etc., have been identified, which appear to play an essential role during implantation [4, 5]. The importance of implantation in reproduction is exemplified by the fact that loss of function of genes such as HOX [6] and COX2 [7], which are essential for implantation, leads to infertility. "
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    ABSTRACT: Luman/CREB3 recruitment factor (LRF or CREBRF) was identified as a regulator of Luman (or CREB3) that is involved in the unfolded protein response during endoplasmic reticulum stress. Luman is implicated in a multitude of functions ranging from viral infection and immunity to cancer. The biological function of LRF, however, is unknown. In this paper, we report that uteri of pregnant mice and embryos displayed enhanced LRF expression at all stages, and the expressed LRF was found to be localized specifically at implantation sites. On the other hand, uteri of mice induced for delayed implantation or pseudopregnant mice showed low levels of LRF expression, suggesting that LRF mediates uterine receptivity during implantation. Further, expression of LRF was found to be modulated by steroid hormones such as progesterone and estradiol. This study thereby identifies a potential role for LRF in the process of implantation in uteri and development of preimplantation embryos in mice.
    Journal of Reproduction and Development 02/2013; 59(3). DOI:10.1262/jrd.2012-137 · 1.52 Impact Factor
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    • "A yet unknown proportion of implantation failure in IVF might be due to yet undiscovered endometrial defects (Horcajadas et al., 2007, 2008; Aghajanova et al., 2008; Oehninger, 2008). But so far, neither the key molecules involved, nor their strict localization and temporal variations are well known and therefore, the possibility for clinical intervention is limited (Dey et al., 2004; Yoshinaga, 2008). Studies focusing on early human implantation have been largely descriptive. "
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    ABSTRACT: There is an urgent need to develop optimized experimental models to examine human implantation. These studies aimed to (i) establish a human endometrium-like three-dimensional (3D) culture system, and (ii) examine the attachment of trophoblast-like Jar spheroids to the culture. In the present work, 3D endometrial cultures were constructed with fibrin-agarose as matrix scaffold, and using epithelial and stromal cells from both human primary cultures and established cell lines. An attachment assay between trophoblast cells and the 3D culture was developed. Epithelial cells (cytokeratin(+)) concentrated on top of the matrix forming a monolayer, and stromal cells (vimentin(+)) resided within the matrix, resembling the normal endometrial structure. The capability of primary epithelial cells to form glands spontaneously was observed. Human trophoblast cells (Jar cells) were hCG(+) by immunostaining, allowed to form spheroids, and confirmed to secrete hCG into the medium. Time-dependent experiments demonstrated a high rate of attachment of Jar spheroids to the epithelium, and adhesion was strongly related to the various cell types present in the 3D culture. An architecturally and functionally competent 3D endometrial culture system was established, that coupled with Jar spheroids mimicking trophoblast cells, provides a unique in vitro model for the study of certain aspects of human implantation.
    Molecular Human Reproduction 01/2012; 18(1):33-43. DOI:10.1093/molehr/gar064 · 3.75 Impact Factor
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