A 3-Hydroxypropionate/4-Hydroxybutyrate Autotrophic Carbon Dioxide Assimilation Pathway in Archaea

Mikrobiologie, Fakultät Biologie, Universität Freiburg, Schänzlestrasse 1, D-79104 Freiburg, Germany.
Science (Impact Factor: 33.61). 07/2008; 318(5857):1782-6. DOI: 10.1126/science.1149976
Source: PubMed


The assimilation of carbon dioxide (CO2) into organic material is quantitatively the most important biosynthetic process. We discovered that an autotrophic member of the archaeal order Sulfolobales, Metallosphaera sedula, fixed CO2 with acetyl-coenzyme A (acetyl-CoA)/propionyl-CoA carboxylase as the key carboxylating enzyme. In this system, one acetyl-CoA and two bicarbonate molecules were reductively converted via 3-hydroxypropionate to succinyl-CoA. This intermediate was reduced to 4-hydroxybutyrate and converted into two acetyl-CoA molecules via 4-hydroxybutyryl-CoA dehydratase. The key genes of this pathway were found not only in Metallosphaera but also in Sulfolobus, Archaeoglobus, and Cenarchaeum species. Moreover, the Global Ocean Sampling database contains half as many 4-hydroxybutyryl-CoA dehydratase sequences as compared with those found for another key photosynthetic CO2-fixing enzyme, ribulose-1,5-bisphosphate carboxylase-oxygenase. This indicates the importance of this enzyme in global carbon cycling.

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    • "Recently discovered in the domain Archaea are the 3-hydroxypropio- nate / 4-hydroxybutyrate cycle (Berg et al., 2007) and the dicarboxylate / 4-hydroxybutyrate cycle (Huber et al., 2008), which are formed, as suggested (Marakushev and Belonogova, 2011) in the later divergent evolution of the original CAF bicycle. Logically, it becomes reasonable to assume that the reduced, non-closed reductive citrate pathway described in heliobacteria (Pickett et al., 1994; Sattley et al., 2008), as well as the reductive acetyl-CoA pathway in methanogenic archaea and acetate-producing clostridia (Ljungdahl and Wood, 1965; Wood, 1991; Ljungdahl, 2009) are also the result of the development of the CAF bicycle. "
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    ABSTRACT: We dedicated our review to the memory of the legendary co-discoverer of the photosynthetic reductive pentose phosphate cycle, Professor Emeritus Andrew Alm Benson, on the occasion of his death at the age of 97, on January 16, 2015. Th is review examines the complexity and diversity of photoreductive carbon metabolism pathways in prokaryotic and eukaryotic phototrophs from the point of view of evolutionary adaptation. In response to global environmental change, the functional signifi cance of adaptive rearrangements in the biochemistry and structure of the photosynthetic apparatus is evaluated. We discuss the possibility of a functional interrelationship between phototrophic and heterotrophic tissues / cells in order to optimize the anabolism in symbiotic organisms, macrophyte algae, and photosynthesizing organs of aquatic and terrestrial higher plants. We also discuss the protective strategy of photosynthetic machinery from the negative infl uence of solar radiation, the positive role of some metal ions in this protective process, and the concept of photohalosynthesis.
    Photosynthesis : New Approaches to the Molecular, Cellular, and Organismal Levels, Edited by S. I. Allakhverdiev, 01/2016: chapter 6: pages 233-326; Scrivener Publishing LLC., ISBN: ISBN 978-1-119-08370-2
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    • "A novel pathway of CO 2 fixation found by Berg et al. (2007) in Archaea. Four other pathways are known by which autotrophic representatives of bacteria, archaea, and eukarya fix carbon "

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    • "The MGI clade has been reported to dominate archaeal communities in the global ocean biosphere (Takai et al., 2006) and in the sea waters surrounding the cooler hydrothermal environments (Takai et al., 2004; Dick and Tebo, 2010; Roussel et al., 2011), which is in accordance with our results. The ammonia-oxidizing members of MGI, mostly represented by Candidatus Nitrosopumilus, presumably undergo the predominant type of chemolithoautotrophy in the dark ocean, via the 3-hydroxypropionate/4-hydroxybutyrate cycle (Berg et al., 2007). Other OTUs affiliated to anammox (anaerobic ammonium oxidation) bacteria from the Nitrosomonas and Nitrosococcus genera. "
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    ABSTRACT: Deep-sea hydrothermal sediments are known to support remarkably diverse microbial consortia. Culture-independent sequence-based technologies have extensively been used to disclose the associated microbial diversity as most of the microorganisms inhabiting these ecosystems remain uncultured. Here we provide the first description of the microbial community diversity found on sediments from Menez Gwen vent system. We compared hydrothermally influenced sediments, retrieved from an active vent chimney at 812 m depth, with non-hydrothermally influenced sediments, from a 1400 m depth bathyal plain. Considering the enriched methane and sulfur composition of Menez Gwen vent fluids, and the sediment physicochemical properties in each sampled area, we hypothesized that the site-associated microbes would be different. To address this question, taxonomic profiles of bacterial, archaeal and micro-eukaryotic representatives were studied by rRNA gene tag pyrosequencing. Communities were shown to be significantly different and segregated by sediment geographical area. Specific mesophilic, thermophilic and hyperthermophilic archaeal (e.g., Archaeoglobus, ANME-1) and bacterial (e.g., Caldithrix, Thermodesulfobacteria) taxa were highly abundant near the vent chimney. In contrast, bathyal-associated members affiliated to more ubiquitous phylogroups from deep-ocean sediments (e.g., Thaumarchaeota MGI, Gamma- and Alphaproteobacteria). This study provides a broader picture of the biological diversity and microbial biogeography, and represents a preliminary approach to the microbial ecology associated with the deep-sea sediments from the Menez Gwen hydrothermal vent field.
    Marine Genomics 09/2015; DOI:10.1016/j.margen.2015.09.001 · 1.79 Impact Factor
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