Article

Gibberellin regulates pollen viability and pollen tube growth in rice.

Bioscience and Biotechnology Center, Nagoya University, Chikusa, Nagoya 464-8601, Japan.
The Plant Cell (Impact Factor: 9.58). 01/2008; 19(12):3876-88. DOI: 10.1105/tpc.107.054759
Source: PubMed

ABSTRACT Gibberellins (GAs) play many biological roles in higher plants. We collected and performed genetic analysis on rice (Oryza sativa) GA-related mutants, including GA-deficient and GA-insensitive mutants. Genetic analysis of the mutants revealed that rice GA-deficient mutations are not transmitted as Mendelian traits to the next generation following self-pollination of F1 heterozygous plants, although GA-insensitive mutations are transmitted normally. To understand these differences in transmission, we examined the effect of GA on microsporogenesis and pollen tube elongation in rice using new GA-deficient and GA-insensitive mutants that produce semifertile flowers. Phenotypic analysis revealed that the GA-deficient mutant reduced pollen elongation1 is defective in pollen tube elongation, resulting in a low fertilization frequency, whereas the GA-insensitive semidominant mutant Slr1-d3 is mainly defective in viable pollen production. Quantitative RT-PCR revealed that GA biosynthesis genes tested whose mutations are transmitted to the next generation at a lower frequency are preferentially expressed after meiosis during pollen development, but expression is absent or very low before the meiosis stage, whereas GA signal-related genes are actively expressed before meiosis. Based on these observations, we predict that the transmission of GA-signaling genes occurs in a sporophytic manner, since the protein products and/or mRNA transcripts of these genes may be introduced into pollen-carrying mutant alleles, whereas GA synthesis genes are transmitted in a gametophytic manner, since these genes are preferentially expressed after meiosis.

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    ABSTRACT: Plant growth and gibberellins (GAs) biosynthesis are two separate but linked processes, involving many genes but fewer have been reported for their role in plant growth and development. Due to little information on the genes involved in such processes, the known plant growth promoting and GAs producing fungal endophyte Penicillium citrinum KACC43900 was used as potential tool to obtain a blueprint of the putative growth promoting and GAs synthesizing proteins. For proteomic analysis, the seedlings of Glehnia littoralis were treated with culture filtrate of P. citrinum KACC43900, which revealed significant differences between 2 dimensional gel electrophoresis profile of the crude protein extracts of the treated and control samples. Matrix-assisted laser desorption/ionization analysis of the 56 selected spots led to the identification of 41 proteins. A significant number (31.5%) of these highly expressed proteins were associated with plant growth regulation, including beta-expansin EXPB4, ent-kaur 16-ene synthase, gibberellin 3-oxidase, and cytochrome P450 family proteins. Proteins involved in regulating energy metabolism and intracellular redox conditions, such as glyceraldehyde-3-phosphate dehydrogenase and ribulose-1,5-bisphospahate carboxylase/oxygenase, were also expressed. It was concluded that culture filtrate of P. citrinum KACC43900 altered the gene expression pattern of host G. littoralis. Current study highlighted the importance of proteomics as a starting tool for any post-genomic research.
    Journal of Plant Interactions 02/2015; 10(1):51-58. DOI:10.1080/17429145.2014.956236 · 0.87 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Plant growth and gibberellins (GAs) biosynthesis are two separate but linked processes, involving many genes but fewer have been reported for their role in plant growth and development. Due to little information on the genes involved in such processes, the known plant growth promoting and GAs producing fungal endophyte Penicillium citrinum KACC43900 was used as potential tool to obtain a blueprint of the putative growth promoting and GAs synthesizing proteins. For proteomic analysis, the seedlings of Glehnia littoralis were treated with culture filtrate of P. citrinum KACC43900, which revealed significant differences between 2 dimensional gel electrophoresis profile of the crude protein extracts of the treated and control samples. Matrix-assisted laser desorption/ionization analysis of the 56 selected spots led to the identification of 41 proteins. A significant number (31.5%) of these highly expressed proteins were associated with plant growth regulation, including beta-expansin EXPB4, ent-kaur 16-ene synthase, gibberellin 3-oxidase, and cytochrome P450 family proteins. Proteins involved in regulating energy metabolism and intracellular redox conditions, such as glyceraldehyde-3-phosphate dehydrogenase and ribulose-1,5-bisphospahate carboxylase/oxygenase, were also expressed. It was concluded that culture filtrate of P. citrinum KACC43900 altered the gene expression pattern of host G. littoralis. Current study highlighted the importance of proteomics as a starting tool for any post-genomic research.
    Journal of Plant Interactions 02/2015; 10(1):51-58. · 0.87 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Plant growth and gibberellins (GAs) biosynthesis are two separate but linked processes, involving many genes but fewer have been reported for their role in plant growth and development. Due to little information on the genes involved in such processes, the known plant growth promoting and GAs producing fungal endophyte Penicillium citrinum KACC43900 was used as potential tool to obtain a blueprint of the putative growth promoting and GAs synthesizing proteins. For proteomic analysis, the seedlings of Glehnia littoralis were treated with culture filtrate of P. citrinum KACC43900, which revealed significant differences between 2 dimensional gel electrophoresis profile of the crude protein extracts of the treated and control samples. Matrix-assisted laser desorption/ionization analysis of the 56 selected spots led to the identification of 41 proteins. A significant number (31.5%) of these highly expressed proteins were associated with plant growth regulation, including beta-expansin EXPB4, ent-kaur 16-ene synthase, gibberellin 3-oxidase, and cytochrome P450 family proteins. Proteins involved in regulating energy metabolism and intracellular redox conditions, such as glyceraldehyde-3-phosphate dehydrogenase and ribulose-1,5-bisphospahate carboxylase/ oxygenase, were also expressed. It was concluded that culture filtrate of P. citrinum KACC43900 altered the gene expression pattern of host G. littoralis. Current study highlighted the importance of proteomics as a starting tool for any post-genomic research.
    Journal of Plant Interactions 03/2015; 10:51–58. · 0.87 Impact Factor

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