New anti-proliferative agent, MK615, from Japanese apricot "Prunus mume" induces striking autophagy in colon cancer cells in vitro.

Second Department of Surgery, Dokkyo University School of Medicine, Kitakobayashi 880, Mibu, Shimotsuga, Tochigi 321-0293, Japan.
World Journal of Gastroenterology (Impact Factor: 2.37). 01/2008; 13(48):6512-7.
Source: PubMed


To investigate the anti-neoplastic effects of MK615, an extract from the Japanese apricot (Prunus mume), against colon cancer cells.
Three colon cancer cell lines, SW480, COLO, and WiDr, were cultured with MK615. Growth inhibition was evaluated by cell proliferation assay and killing activity was determined by lactate dehydrogenase assay. Induction of apoptosis was evaluated by annexin V flow cytometry. Morphological changes were studied by light and electron microscopy, and immunofluorescence staining with Atg8.
MK615 inhibited growth and lysed SW480, COLO and WiDr cells in a dose-dependent manner. Annexin V flow cytometry showed that MK615 induced apoptosis after 6 h incubation, at which point the occurrence of apoptotic cells was 68.0%, 65.7% and 64.7% for SW480, COLO, and WiDr cells, respectively. Light and electron microscopy, and immunofluorescence staining with Atg8 revealed that MK615 induced massive cytoplasmic vacuoles (autophagosomes) in all three cell lines.
MK615 has an anti-neoplastic effect against colon cancer cells. The effect may be exerted by induction of apoptosis and autophagy.

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    • "It has been a traditional Japanese medicine for centuries and is a familiar and commonly consum ed food. The health benefits of Ume are now being widely recognized and have been strengthened by recent studies showing that MK615, an extract of compounds from Ume, has various pharmacological activities that include a potential source of free radical scavenging, antiviral, antimicrobial, anti-inflammatory and anticancer activities (Jeong et al., 2006; Adachi et al., 2007; Mori et al., 2007; Nakagawa et al., 2007; Okada et al., 2008; Morimoto et al., 2009; Enomoto et al., 2010; Chung et al., 2011; Morimoto-Yamashita et al., 2011; Park et al., 2011). "
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    • "It contains several triterpenoids and has been shown to exert an anti-neoplastic effect against human cancers, such as breast cancer, stomach cancer, hepatocellular carcinoma, colon cancer and malignant melanoma. The mechanisms responsible for the anti-neoplastic effects of MK615 include induction of apoptosis and autophagy, and suppression of Aurora A kinase in cancer cells (Nakagawa et al., 2007; Adachi et al., 2007; Okada et al., 2007; Mori et al., 2007; Matsushita et al., 2010). 3. Inhibitory effects of an extract from Ume on Helicobacter pylori Helicobacter pylori infection is an important factor in human gastric disorders, including chronic active gastritis, peptic ulcers, intestinal metaplasia and cancer. "
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