Selective Fluorescent Chemosensor for the Bacterial Alarmone (p)ppGpp

Department of Chemistry, College of Natural Sciences, Seoul National University, Seoul 151-747, Korea.
Journal of the American Chemical Society (Impact Factor: 12.11). 02/2008; 130(3):784-5. DOI: 10.1021/ja0759139
Source: PubMed


We have developed the first selective fluorescent chemosensor (PyDPA) for (p)ppGpp, a bacterial and plant alarmone. By using pyrene-excimer fluorescence, PyDPA shows very good selectivity for (p)ppGpp from among other nucleotides in water. PyDPA was used for the real-time detection of in vitro ppGpp synthesis by bacterial ribosomal complexes.

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