Homophilic Interactions of the Amyloid Precursor Protein (APP) Ectodomain Are Regulated by the Loop Region and Affect -Secretase Cleavage of APP

Institut für Chemie und Biochemie, Freie Universität Berlin, 14195 Berlin, Germany.
Journal of Biological Chemistry (Impact Factor: 4.57). 04/2008; 283(11):7271-9. DOI: 10.1074/jbc.M708046200
Source: PubMed


We found previously by fluorescence resonance energy transfer experiments that amyloid precursor protein (APP) homodimerizes in living cells. APP homodimerization is likely to be mediated by two sites of the ectodomain and a third site within the transmembrane sequence of APP. We have now investigated the role of the N-terminal growth factor-like domain in APP dimerization by NMR, biochemical, and cell biological approaches. Under nonreducing conditions, the N-terminal domain of APP formed SDS-labile and SDS-stable complexes. The presence of SDS was sufficient to convert native APP dimers entirely into monomers. Addition of an excess of a synthetic peptide (APP residues 91-116) containing the disulfide bridge-stabilized loop inhibited cross-linking of pre-existing SDS-labile APP ectodomain dimers. Surface plasmon resonance analysis revealed that this peptide specifically bound to the N-terminal domain of APP and that binding was entirely dependent on the oxidation of the thiol groups. By solution-state NMR we detected small chemical shift changes indicating that the loop peptide interacted with a large protein surface rather than binding to a defined pocket. Finally, we studied the effect of the loop peptide added to the medium of living cells. Whereas the levels of alpha-secretory APP increased, soluble beta-cleaved APP levels decreased. Because Abeta40 and Abeta42 decreased to similar levels as soluble beta-cleaved APP, we conclude either that beta-secretase binding to APP was impaired or that the peptide allosterically affected APP processing. We suggest that APP acquires a loop-mediated homodimeric state that is further stabilized by interactions of hydrophobic residues of neighboring domains.

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    • "The Aβ segment of APP is another region that directly interacts with copper and is explained in more detail in Section " Metal Modulation of Aβ Generation, Aggregation, and Cell Toxicity. " The N-terminal copper binding domain of APP has been found to play crucial roles in homodimerization (Hesse et al., 1994; Kaden et al., 2008), and an elevation in copper levels increases APP homodimerization (Noda et al., 2013). Zinc binds to a conserved region of amino acids between position 170 and 188 of APP (Bush et al., 1993, 1994a). "
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    ABSTRACT: Alzheimer's disease (AD) is a multifactorial neurodegenerative disease. It begins years prior to the onset of clinical symptoms, such as memory loss and cognitive decline. Pathological hallmarks of AD include the accumulation of β-amyloid in plaques and hyperphosphorylated tau in neurofibrillary tangles. Copper, iron, and zinc are abnormally accumulated and distributed in the aging brain. These metal ions can adversely contribute to the progression of AD. Dysregulation of cholesterol metabolism has also been implicated in the development of AD pathology. To date, large bodies of research have been carried out independently to elucidate the role of metals or cholesterol on AD pathology. Interestingly, metals and cholesterol affect parallel molecular and biochemical pathways involved in AD pathology. The possible links between metal dyshomeostasis and altered brain cholesterol metabolism in AD are reviewed.
    Frontiers in Aging Neuroscience 05/2014; 6:91. DOI:10.3389/fnagi.2014.00091 · 4.00 Impact Factor
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    • "It is yet to be determined the functional effects mediated by homo-and heterodimerisation of the APP family members, although it has been hypothesized that cis-dimerisation of membrane bound APP with sAPPa may allow activation of intracellular signalling pathways (Gralle et al. 2009; Reinhard et al. 2005). Alternatively cis dimerisation may simply influence the processing of APP, possibly by changing its conformational state, thereby decreasing its amyloidogenic processing by preventing access of b-secretase to its cleavage site (Kaden et al. 2008). It is possible that sAPPa may act in a similar manner to known growth factors, whereby heparin is required to facilitate binding of APP to a co-receptor, through the APP96-110 region. "
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    ABSTRACT: We have previously shown that following traumatic brain injury (TBI) the presence of the amyloid precursor protein (APP) may be neuroprotective. APP knockout mice have increased neuronal death and worse cognitive and motor outcomes following TBI, which is rescued by treatment with exogenous sAPPα (the secreted ectodomain of APP generated by α-secretase cleavage). Two neuroprotective regions were identified in sAPPα, the N and C-terminal domains D1 and D6a/E2 respectively. As both D1 and D6a/E2 contain heparin binding activity it was hypothesised that this is responsible for the neuroprotective activity. In this study we focused on the heparin binding site, encompassed by residues 96-110 in D1, which has previously been shown to have neurotrophic properties. We found that treatment with APP96-110 rescued motor and cognitive deficits in APP-/- mice following focal TBI. APP96-110 also provided neuroprotection in Sprague Dawley rats following diffuse TBI. Treatment with APP96-110 significantly improved functional outcome as well as preserve histological cellular morphology in APP-/- mice following focal controlled cortical impact injury. Furthermore, following administration of APP96-110 in rats after diffuse impact acceleration TBI, motor and cognitive outcomes were significantly improved and axonal injury reduced. These data define the heparin binding site in the D1 domain of sAPPα, represented by the sequence APP96-110, as the neuroprotective site to confer neuroprotection following TBI. This article is protected by copyright. All rights reserved.
    Journal of Neurochemistry 08/2013; 128(1). DOI:10.1111/jnc.12391 · 4.28 Impact Factor
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    • "Moreover, binding of heparin to E1eE2 domains also promotes APP dimerization (Dahms et al., 2010; Gralle et al., 2006). Providing a physiological consequence for these interactions, APP dimerization was shown to affect its processing by secretases (Kaden et al., 2008; Lefort et al., 2012; Munter et al., 2007; Scheuermann et al., 2001). Because the extracellular portion of APP contains various structural domains, it is not surprising that several putative physiological ligands have been identified for APP. "
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