Article

Site-specific N-terminal labelling of proteins in vitro and in vivo using N-myristoyl transferase and bioorthogonal ligation chemistry.

Department of Chemistry, South Kensington Campus, Imperial College, London, UK.
Chemical Communications (impact factor: 6.17). 02/2008; DOI:10.1039/b716115h pp.480-2
Source: PubMed

ABSTRACT N-Myristoyl transferase-mediated modification with azide-bearing substrates is introduced as a highly selective and practical method for in vitro and in vivo N-terminal labelling of a recombinant protein using bioorthogonal ligation chemistry.

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Keywords

bioorthogonal ligation chemistry
 
N-Myristoyl transferase-mediated modification
 
practical method
 
vivo N-terminal labelling