High-density mapping of single-molecule trajectories with photoactivated localization microscopy.

National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.
Nature Methods (Impact Factor: 25.95). 03/2008; 5(2):155-7. DOI: 10.1038/nmeth.1176
Source: PubMed

ABSTRACT We combined photoactivated localization microscopy (PALM) with live-cell single-particle tracking to create a new method termed sptPALM. We created spatially resolved maps of single-molecule motions by imaging the membrane proteins Gag and VSVG, and obtained several orders of magnitude more trajectories per cell than traditional single-particle tracking enables. By probing distinct subsets of molecules, sptPALM can provide insight into the origins of spatial and temporal heterogeneities in membranes.

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