Separation of diastereomeric anthrone-C-glucosyls of aloes by micellar electrokinetic capillary chromatography

Lehrstuhl Pharmazeutische Biologie, Fakultät für Biowissenschaften, Pharmazie und Psychologie, Universität Leipzig, Leipzig, Germany.
Pharmazie (Impact Factor: 1.05). 01/2008; 62(12):955-7. DOI: 10.1691/ph.2007.12.7677
Source: PubMed


The anthrone-C-glucosyls aloin A and B, 5-hydroxyaloin A, 10-hydroxyaloin A and B were separated by micellar electrokinetic capillary chromatography (MECC) with sodium dodecyl sulfate in borate buffer pH 9 within less than 7 min. The method has been successfully transferred to the analysis of the two European Pharmacopoeia drugs Cape aloes and Curaçao aloes. A comparison of the peak areas received by HPLC and MECC indicated the transferability of the measured contents.

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    ABSTRACT: Natural products such as pure phytoconstituents and plant extracts offer limitless opportunities for the new drug development due to its unmatched chemical diversity. Plants play an important role in the medicinal preparations for both preventive and curative purpose. Some of the currently available drugs i.e. aspirin, digitalis, anti-malarial (quinine) and anti-cancer (vincristine, vinblastine) were derived from the plant sources. Aloin (C21H22O9), a yellow colour compound is a mixture of two diastereoisomers, aloin A and aloin B. Aloin is an anthrone C-glucoside having molecular weight 418, and it is the main phytoconstituents of aloes. Aloin is used for various pharmacological purposes such as laxative agent. It is also used as ingredients of various laxative pharmaceutical preparations. So far, varieties of analytical methods have been developed for the estimation of aloin in aloes product, which are mainly based on HPLC and TLC techniques. In the present review, pharmacological activities and analytical aspects of aloin were highlighted along with some useful tissue culture techniques. This review could be helpful to the researcher for the investigation of new molecule from aloin in the future.
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