The potential of mannosylated chitosan microspheres to target macrophage mannose receptors in an adjuvant-delivery system for intranasal immunization

Department of Agricultural Biotechnology, Research Institute for Agriculture and Life Sciences, Seoul National University, Seoul, Republic of Korea.
Biomaterials (Impact Factor: 8.56). 05/2008; 29(12):1931-9. DOI: 10.1016/j.biomaterials.2007.12.025
Source: PubMed


A vaccine delivery system based on mannosylated chitosan microspheres (MCMs) was studied in vitro and in vivo. Bordetella bronchiseptica antigens containing dermonecrotoxin (BBD) were loaded in MCMs or chitosan microspheres (CMs). Fluorescence confocal microscopy indicated that BBD-loaded MCMs (BBD-MCMs) bound with mannose receptors on murine macrophages (RAW264.7 cells). In vitro experiments using macrophages demonstrated that BBD-MCMs had more effective immune-stimulating activity than BBD-loaded CMs (BBD-CMs). Mice intranasally immunized with BBD-MCMs showed significantly higher BBD-specific IgA antibody responses in saliva and serum than mice immunized with BBD-CMs (p<0.05). After challenge with B. bronchiseptica via the nasal cavity, groups treated with BBD-MCMs or BBD-CMs showed similar patterns with a high survival rate even though there was no significant difference between those groups. These results suggested that mannose moieties in the MCMs enhanced immune-stimulating activities through mucosal delivery due to a specific interaction between mannose groups in the MCMs and mannose receptors on the macrophages.

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    • "Non-viral methods of gene delivery, including cationic polymers and lipids that can form polyplexes with DNA vaccines are another promising approach, since DNA polyplexes are efficiently protected from enzymatic degradation and exhibit increased cell transfection efficiency [8] [9]. Likewise, cationic polymer carriers containing mannose moieties have been reported to mediate targeted DNA delivery to professional antigen presenting cells (APCs), including macrophages and dendritic cells [10] [11]. However, cationic polymer-based DNA vaccines still require a trained healthcare professional to perform the injection with a hyperdermic syringe. "
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    Journal of Controlled Release 01/2014; 179(1). DOI:10.1016/j.jconrel.2014.01.016 · 7.71 Impact Factor
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    • "In a recent study, nine ILs (differing by their ion type or chain length on the cation) have been screened for the same synthesis whilst 1-butyl- 1-methylpyrrolidinium trifluoromethanesulfonate [Bmpyrr][TFO] was highlighted as the best in terms of yield and reaction rate [20]. These studies focus on mannose esters because mannose vaccine or drug carrier can be interesting for increasing immunogenicity [21] [22] and tumor targeting [23]. As for the choice of the acyl moiety, molecules based on myristic acid are used to modify proteins by myristoylation for protein-membrane binding and protein-protein interactions improvement [24] [25]. "
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    Process Biochemistry 08/2013; 48(12). DOI:10.1016/j.procbio.2013.08.023 · 2.52 Impact Factor
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    • "Thus, the problem of inefficient delivery for the plasmid vector must be addressed before achieving a better outcome for the DNA vaccine. It is well known that APCs, such as dendritic cells (DCs), express a large amount of mannose receptor on the surface.14 To enhance the specificity of drug delivery, a large number of research papers have explored different mannosylated drug delivery systems, such as mannosylated chitosan,14 mannosylated liposome,15 and mannosylated polyethyleneimine 25k (PEI 25k). "
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    International Journal of Nanomedicine 05/2013; 8:1843-1854. DOI:10.2147/IJN.S43827 · 4.38 Impact Factor
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