Article

Real-time monitoring approach: assessment of effects of antibodies on the adhesion of NCI-H460 cancer cells to the extracellular matrix.

Institute of Microelectronics, 11 Science Park Road, Singapore Science Park II, Singapore 117685, Singapore.
Biosensors and Bioelectronics (impact factor: 5.6). 05/2008; 23(9):1390-6. DOI:10.1016/j.bios.2007.12.006
Source: PubMed

ABSTRACT This paper presents an in situ impedance chip system, which allows studying the effects of drugs on the behaviors of living cell in real-time. A new label-free measurement approach is introduced, which enables to assess the extent of the adhesion of a cell population to the extracellular matrix (ECM)-coated microelectrode array, by adding together all the impedance changes from individually controlled microelectrodes. A high sensitivity was demonstrated and allowed us to monitor cell attachment with the resolution of a "few cells". The dose and pre-incubation time effects of antibodies against beta1-integrin and its subunit alpha2beta1-integrin on the adhesion behavior of NCI-H460 lung cancer cells to collagen type I was extensively studied. Results show that both anti-beta1-integrin and anti-alpha2beta1-integrin inhibit NCI-H460 cells attachment to collagen I. This indicates that beta1-integrin is present on the surface of NCI-H460 cancer cells, and that its subunit alpha2beta1 significantly affects NCI-H460 cells attachment on collagen I.

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    Article: Trends in cell-based electrochemical biosensors.
    Lin Ding, Dan Du, Xueji Zhang, Huangxian Ju
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    ABSTRACT: Cell-based electrochemical biosensors have contributed tremendously to the fields of biology, medicine, chemistry, pharmacology, and environmental science. With electrochemical transducers and whole cells as the recognition elements, these biosensors provide new horizons for biosensing and life science research. This review focuses on the research accomplishments on this topic over the last three years, and is divided into three sections according to the types of cellular responses. Our aim is to highlight how simple and sensitive electrochemical methods can be coupled with cells by virtue of the integration of interface control, nanotechnology and genetic engineering to generate new enabling technologies. Some specific examples to demonstrate how these sensors are useful in medicinal chemistry and drug design have also been discussed. It is hoped that this review can provide inspiration for the development of fast, selective, sensitive, and convenient detection and diagnosis platforms.
    Current Medicinal Chemistry 02/2008; 15(30):3160-70. · 4.86 Impact Factor
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Keywords

adhesion behavior
 
anti-alpha2beta1-integrin
 
behaviors
 
cell attachment
 
cell population
 
collagen I
 
collagen type
 
drugs
 
ECM)-coated microelectrode array
 
extracellular matrix
 
impedance changes
 
NCI-H460 cancer cells
 
NCI-H460 cells attachment
 
NCI-H460 lung cancer cells
 
new label-free measurement approach
 
paper presents
 
pre-incubation time effects
 
real-time
 
situ impedance chip system
 
subunit alpha2beta1-integrin