Article

Osteogenic differentiation of human mesenchymal stem cells on chargeable polymer-modified surfaces.

National Engineering Research Center for Biomaterials, Sichuan University, 29 Wangjiang Road, Chengdu 610064, China.
Journal of Biomedical Materials Research Part A (impact factor: 2.63). 02/2008; 87(4):903-12. DOI:10.1002/jbm.a.31834 pp.903-12
Source: PubMed

ABSTRACT Polystyrene cell-culture plates modified with positively charged polyallylamine (PAAm) and negatively charged poly(acrylic acid) (PAAc) and unmodified plate were used for the culture of human mesenchymal stem cells (MSCs) to study the effect of surface electrostatic properties on their osteogenic differentiation. All of these surfaces supported cell adhesion and proliferation. However, the cells adhered, spread, and proliferated somewhat more quickly on the PAAm-modified surface than they did on the PAAc-modified and control surfaces. Osteogenic differentiation was examined by alkaline phosphatase (ALP) staining, alizarin red S staining, and gene-expression analysis. The MSCs cultured on the three kinds of surfaces in the presence of dexamethasone were positively stained with ALP and alizarin red S staining, while the cells cultured without dexamethasone were not positively stained. Gene-expression analyses using real-time PCR indicated that MSCs cultured on these surfaces in the presence of dexamethasone expressed osteogenic marker genes, encoding ALP, osteocalcin, bone sialoprotein, osteopontin, and type I collagen. These results indicate that the positively charged, negatively charged, and unmodified surfaces supported osteogenic differentiation, and that their effect required the synergistic effect of dexamethasone.

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Keywords

alizarin red S staining
 
alkaline phosphatase
 
cell adhesion
 
cells adhered
 
cells cultured
 
control surfaces
 
encoding ALP
 
Gene-expression analyses
 
gene-expression analysis
 
MSCs cultured
 
Osteogenic differentiation
 
osteogenic marker genes
 
PAAm-modified surface
 
Polystyrene cell-culture plates
 
surface electrostatic properties
 
surfaces
 
synergistic effect
 
three kinds
 
unmodified plate
 
unmodified surfaces
 

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