Article

Semiconductor nanocrystal probes for human metaphase chromosomes.

GeoCenters, Inc. and DNA Technologies Group, National Institute of Standards and Technology, Gaithersburg, MD 20899-8311, USA .
Nucleic Acids Research (impact factor: 8.03). 02/2004; 32(3):e28. DOI:10.1093/nar/gnh024
Source: PubMed

ABSTRACT To improve signal stability and quantitation, an optically stable, novel class of fluorophore for hybridization analysis of human metaphase chromosomes is demonstrated. Detection of hybridization sites in situ was based on fluorescence from streptavidin-linked inorganic crystals of cadmium selenide [(CdSe)ZnS]. Fluorescence of nanocrystal fluorophores was significantly brighter and more photostable than organic fluorophores Texas Red and fluorescein. Thus, semiconductor nanocrystal fluorophores offer a more stable and quantitative mode of fluorescence in situ hybridization (FISH) for research and clinical applications.

0 0
 · 
0 Bookmarks
 · 
31 Views
  • Source
    Article: Semiconductor clusters, nanocrystals, and quantum dots
    A. P. Alivisatos
    [show abstract] [hide abstract]
    ABSTRACT: Current research into semiconductor clusters is focused on the properties of quantum dots-fragments of semiconductor consisting of hundreds to many thousands of atoms-with the bulk bonding geometry and with surface states eliminated by enclosure in a material that has a larger band gap. Quantum dots exhibit strongly size-dependent optical and electrical properties. The ability to join the dots into complex assemblies creates many opportunities for scientific discovery.
    Science. 271(5251):933-937.
  • Source
    Article: Luminescence Photophysics in Semiconductor Nanocrystals
    Manoj Nirmal, Louis Brus
    11/1998;
  • Article: Quantum dot bioconjugates for ultrasensitive nonisotopic detection.
    W C Chan, S Nie
    [show abstract] [hide abstract]
    ABSTRACT: Highly luminescent semiconductor quantum dots (zinc sulfide-capped cadmium selenide) have been covalently coupled to biomolecules for use in ultrasensitive biological detection. In comparison with organic dyes such as rhodamine, this class of luminescent labels is 20 times as bright, 100 times as stable against photobleaching, and one-third as wide in spectral linewidth. These nanometer-sized conjugates are water-soluble and biocompatible. Quantum dots that were labeled with the protein transferrin underwent receptor-mediated endocytosis in cultured HeLa cells, and those dots that were labeled with immunomolecules recognized specific antibodies or antigens.
    Science 10/1998; 281(5385):2016-8. · 31.20 Impact Factor

Show more

Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.

Full-text

View
0 Downloads
Available from

Keywords

cadmium selenide [(CdSe)ZnS]
 
fluorescence
 
fluorophore
 
human metaphase chromosomes
 
nanocrystal fluorophores
 
organic fluorophores Texas Red
 
photostable
 
quantitative mode
 
signal stability
 
streptavidin-linked inorganic crystals
 

Yan Xiao