Article

Harmonic holography: a new holographic principle.

Department of Electrical Engineering, California Institute of Technology, Pasadena, California 91125, USA.
Applied Optics (Impact Factor: 1.69). 03/2008; 47(4):A103-10. DOI: 10.1364/AO.47.00A103
Source: PubMed

ABSTRACT The process of second harmonic generation (SHG) has a unique property of forming a sharp optical contrast between noncentrosymmetric crystalline materials and other types of material, which is a highly valuable asset for contrast microscopy. The coherent signal obtained through SHG also allows for the recording of holograms at high spatial and temporal resolution, enabling whole-field four-dimensional microscopy for highly dynamic microsystems and nanosystems. Here we describe a new holographic principle, harmonic holography (H(2)), which records holograms between independently generated second harmonic signals and reference. We experimentally demonstrate this technique with digital holographic recording of second harmonic signals upconverted from an ensemble of second harmonic generating nanocrystal clusters under femtosecond laser excitation. Our results show that harmonic holography is uniquely suited for ultrafast four-dimensional contrast microscopy.

1 Bookmark
 · 
213 Views
  • [Show abstract] [Hide abstract]
    ABSTRACT: Optical microscopy has played a critical role for discovery in biomedical sciences since Hooke's introduction of the compound microscope. Recent years have witnessed explosive growth in optical microscopy tools and techniques. Information in microscopy is garnered through contrast mechanisms, usually absorption, scattering, or phase shifts introduced by spatial structure in the sample. The emergence of nonlinear optical contrast mechanisms reveals new information from biological specimens. However, the intensity dependence of nonlinear interactions leads to weak signals, preventing the observation of high-speed dynamics in the 3D context of biological samples. Here, we show that for second harmonic generation imaging, we can increase the 3D volume imaging speed from sub-Hertz speeds to rates in excess of 1,500 volumes imaged per second. This transformational capability is possible by exploiting coherent scattering of second harmonic light from an entire specimen volume, enabling new observational capabilities in biological systems.
    Proceedings of the National Academy of Sciences 10/2013; · 9.74 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: In this paper, the detection of a small reflector in a randomly heterogenous medium using second-harmonic generation is investigated. The medium is illuminated by a time-harmonic plane wave at frequency omega. It is assumed that the reflector has a non-zero second-order nonlinear susceptibility, and thus emits a wave at frequency two omega in addition to the fundamental frequency linear scattering. It is shown how the fundamental frequency signal and the second-harmonic signal propagate in the medium. A statistical study of the images obtained by migrating the boundary data is performed. It is proved that the second-harmonic image is more stable with respect to medium noise than the one obtained with the fundamental signal. Moreover, the signal-to-noise ratio for the second-harmonic image does not depend neither on the second-order susceptibility tensor nor on the volume of the particle.
    06/2013;
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: We tackle the problem of information recovery and imaging through scattering microfluidic chips by means of digital holography (DH). In many cases the chip can become opalescent due to residual deposits settling down the inner channel faces, biofilm formation, scattering particle uptake by the channel cladding or its damaging by corrosive substances, or even by condensing effect on the exterior channels walls. In these cases white-light imaging is severely degraded and no information is obtainable at all about the flowing samples. Here we investigate the problem of counting and estimating velocity of cells flowing inside a scattering chip. Moreover we propose and test a method based on the recording of multiple digital holograms to retrieve improved phase-contrast images despite the strong scattering effect. This method helps, thanks to DH, to recover information which, otherwise, would be completely lost.
    Optics Express 10/2013; 21(20):23985-23996. · 3.55 Impact Factor

Full-text (2 Sources)

View
36 Downloads
Available from
May 20, 2014