Central venous catheter-associated Leifsonia aquatica bacteremia in a hemodialysis-dependent patient

Department of Medicine, The Ottawa Hospital General Campus, Ontario, Canada K1H 8L6.
Diagnostic Microbiology and Infectious Disease (Impact Factor: 2.46). 06/2008; 61(1):64-6. DOI: 10.1016/j.diagmicrobio.2007.12.012
Source: PubMed

ABSTRACT Infections associated with Leifsonia aquatica are particularly uncommon. We describe a central venous catheter-associated L. aquatica bacteremia in a hemodialysis-dependent patient. A review of the literature revealed only 1 other case report involving 10 hemodialysis patients with documented L. aquatica bacteremia.

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    • "Leifsonia sp. is an aquatic coryneform rod rarely associated with human infections. Some species, like Leifsonia aquatica, have been accidentally found in the clinic in other countries, but no species previously reported in China [27-29]. We report two cases of bloodstream infection in a female patient with osteogenic sarcoma and a 45-day-old infant. "
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    ABSTRACT: Clinical microbiology laboratories have to accurately identify clinical microbes. However, some isolates are difficult to identify by the automated biochemical text platforms, which are called "difficult-to-identify" microbes in this study. Therefore, the ability of 16S ribosomal DNA (16S rDNA) and internal transcribed spacer 2 (ITS2) sequencing to identify these "difficult-to-identify" bacteria and fungi was assessed in this study. Samples obtained from a teaching hospital over the past three years were examined. The 16S rDNA of four standard strains, 18 clinical common isolates, and 47 "difficult-to-identify" clinical bacteria were amplified by PCR and sequenced. The ITS2 of eight standard strains and 31 "difficult-to-identify" clinical fungi were also amplified by PCR and sequenced. The sequences of 16S rDNA and ITS2 were compared to reference data available in GenBank by using the BLASTN program. These microbes were identified according to the percentage of similarity to reference sequences of strains in GenBank. The results from molecular sequencing methods correlated well with automated microbiological identification systems for common clinical isolates. Sequencing results of the standard strains were consistent with their known phenotype. Overall, 47 "difficult-to-identify" clinical bacteria were identified as 35 genera or species by sequence analysis (with 10 of these identified isolates first reported in clinical specimens in China and two first identified in the international literature). 31 "difficult-to-identify" clinical fungi tested could be identified as 15 genera or species by sequence analysis (with two of these first reported in China). Our results show the importance of 16S rDNA and internal ITS2 sequencing for the molecular identification of "difficult-to-identify" bacteria and fungi. The development of this method with advantages of convenience, availability, and cost-effectiveness will make it worth extending into clinical practice in developing countries.
    Annals of Clinical Microbiology and Antimicrobials 01/2014; 13(1):1. DOI:10.1186/1476-0711-13-1 · 2.19 Impact Factor
    • "Leifsonia aquatica is a non-spore-forming Gram-positive rod that is typically found in environmental water habitats (Funke et al., 1997; Suzuki et al., 1999; Evtushenko et al., 2000). It was first described by Leifson (1962) and has been isolated from samples of distilled water, from municipal water supplies and water from private wells (Sulpher et al., 2008; Beckwith et al., 1986). Although the species was classified under the genus Corynebacterium for almost 40 years, it has recently been reclassified because of its chemotaxonomic and genetic differences to corynebacteria (Suzuki et al., 1999; Funke & Bernard, 2007). "
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    ABSTRACT: Leifsonia aquatica is an aquatic coryneform rod that is capable of forming biofilms in environmental water sources. It has rarely been associated with human infections and its pathogenicity and clinical significance are uncertain. We describe a case of catheter-related bloodstream infection in a haemodialysis patient. The isolate grew on conventional media as a yellow-pigmented colony, but identification required molecular methods. Although the strain displayed reduced sensitivity to vancomycin, the clinical outcome was favourable after catheter removal and intravenous treatment with this antibiotic. Our report gives further evidence of the capability of this aquatic bacterium to cause human infection.
    Journal of Medical Microbiology 03/2012; 61(Pt 6):868-73. DOI:10.1099/jmm.0.037457-0 · 2.25 Impact Factor
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    ABSTRACT: Catalase-positive Gram-positive bacilli, commonly called "diphtheroids" or "coryneform" bacteria were historically nearly always dismissed as contaminants when recovered from patients, but increasingly have been implicated as the cause of significant infections. These taxa have been underreported, and the taxa were taxonomically confusing. The mechanisms of pathogenesis, especially for newly described taxa, were rarely studied. Antibiotic susceptibility data were relatively scant. In this minireview, clinical relevance, phenotypic and genetic identification methods, matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) evaluations, and antimicrobial susceptibility testing involving species in the genus Corynebacterium and other medically relevant Gram-positive rods, collectively called coryneforms, are described.
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