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Bartlett, N.W. et al. Mouse models of rhinovirus-induced disease and exacerbation of allergic airway inflammation. Nat. Med. 14, 199-204

Department of Respiratory Medicine, UK National Heart and Lung Institute, and Medical Research Council & Asthma UK Centre in Allergic Mechanisms of Asthma, Imperial College London, Norfolk Place, London W2 1PG, UK.
Nature medicine (Impact Factor: 28.05). 03/2008; 14(2):199-204. DOI: 10.1038/nm1713
Source: PubMed

ABSTRACT Rhinoviruses cause serious morbidity and mortality as the major etiological agents of asthma exacerbations and the common cold. A major obstacle to understanding disease pathogenesis and to the development of effective therapies has been the lack of a small-animal model for rhinovirus infection. Of the 100 known rhinovirus serotypes, 90% (the major group) use human intercellular adhesion molecule-1 (ICAM-1) as their cellular receptor and do not bind mouse ICAM-1; the remaining 10% (the minor group) use a member of the low-density lipoprotein receptor family and can bind the mouse counterpart. Here we describe three novel mouse models of rhinovirus infection: minor-group rhinovirus infection of BALB/c mice, major-group rhinovirus infection of transgenic BALB/c mice expressing a mouse-human ICAM-1 chimera and rhinovirus-induced exacerbation of allergic airway inflammation. These models have features similar to those observed in rhinovirus infection in humans, including augmentation of allergic airway inflammation, and will be useful in the development of future therapies for colds and asthma exacerbations.

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    • "SPLUNC1 KO and control mice (8–12 weeks old) were anesthetized by intra-peritoneal (i.p.) injection of ketamine (80 mg/kg) and xylazine (10 mg/kg), and intranasally inoculated with HRV-1B at 5×106 pfu/mouse as HRV-1B is the only HRV that can directly infect mouse lungs [32]. After 24 h, the left lung was homogenized for examining HRV RNA levels by quantitative real-time RT-PCR. "
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    PLoS ONE 09/2014; 9(9):e108342. DOI:10.1371/journal.pone.0108342 · 3.23 Impact Factor
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    • "Another potential concern relates to our model’s dependence on minor group viruses. However, effects of RV1B infection on wild-type mice are indistinguishable from those of RV16, a major group virus, on transgenic human ICAM-1 mice [63]. Major and minor group viruses induce nearly identical patterns of gene expression in cultured airway epithelial cells [64]. "
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    • "While there was also a trend towards increased sensitivity to MCh for Raw, Rrs, G and H, these were not significant (Figure 6). The additive effect of the two insults on BAL neutrophilia is commonly seen in similar studies employing ovalbumin [15], [16], [17]. Further, neutrophils are found in greater numbers in the nasal secretions, sputum, and BAL of allergic humans experimentally infected with un-purified HRV-16, compared with healthy controls [46], [47]. "
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    PLoS ONE 03/2014; 9(3):e92163. DOI:10.1371/journal.pone.0092163 · 3.23 Impact Factor
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