Direct Evidence for Epithelial-Mesenchymal Transitions in Breast Cancer

Department of Molecular Genetics, College of Medicine, The Ohio State University, Columbus, OH 43210, USA.
Cancer Research (Impact Factor: 9.33). 03/2008; 68(3):937-45. DOI: 10.1158/0008-5472.CAN-07-2148
Source: PubMed


We developed stromal- and epithelial-specific cre-transgenic mice to directly visualize epithelial-mesenchymal transition (EMT) during cancer progression in vivo. Using three different oncogene-driven mouse mammary tumor models and cell-fate mapping strategies, we show in vivo evidence for the existence of EMT in breast cancer and show that myc can specifically elicit this process. Hierarchical cluster analysis of genome-wide loss of heterozygosity reveals that the incidence of EMT in invasive human breast carcinomas is rare, but when it occurs it is associated with the amplification of MYC. These data provide the first direct evidence for EMT in breast cancer and suggest that its development is favored by myc-initiated events.

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Available from: Michael C Ostrowski, Oct 05, 2015
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    • "Also, cancer cell migration and invasion were comparable (Figures 2E–2L). Since previous studies documented only minimal EMT in MMTV-PyMT tumors (Trimboli et al., 2008), we investigated other mechanisms via which PHD2 haplodeficiency reduced intravasation and dissemination. PHD2 Haplodeficiency in Cancer Cells Does Not Affect Cancer-Cell-Intrinsic Invasive Properties but Reduces Metastasis To assess the role of PHD2 in cancer cells, we generated mice in which one PHD2 allele was deleted in cancer cells by intercrossing PHD2 +/lox mice with MMTV-Cre mice and then with "
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    Cell Reports 07/2015; 12(6). DOI:10.1016/j.celrep.2015.07.010 · 8.36 Impact Factor
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    • "In vitro studies have also shown NEU to act as an active rat mammary gland genotoxic carcinogen [4,49]. During the process of neoplastic transformation, one of the earliest stages of invasion is epithelial to mesenchymal transition (EMT) wherein the epithelial cells acquire mesenchymal characteristic so as to invade the surrounding extracellular matrix and migrate towards distant organs [50,51]. EMT is characterised by loss of polarity of the epithelial cells, appearance of mesenchymal markers (upregulation of vimentin, fibronectin, N-cadherin) and down regulation of the epithelial markers (E-cadherin, occludins, cytokeratin 19, claudins) [52,53]. "
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    BMC Cancer 04/2014; 14(1):287. DOI:10.1186/1471-2407-14-287 · 3.36 Impact Factor
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    • "Epithelial-originated cancer cells, such as breast cancer cells, transition between phases of epithelial and mesenchymal cells during pathological progression [1], [2]. Initially, cancer cells transit from epithelial cells to mesenchymal cells through epithelial-mesenchymal transition (EMT), which is a critical step required for metastasis. "
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