Anti- inflammatory and analgesic properties of the leaf extracts and essential oil of Lavandula angustifolia mill. J Ethnopharmacol

Department of Pharmacology, School of Pharmacy and Pharmaceutical Sciences, Isfahan University of Medical Sciences, Isfahan, Iran.
Journal of Ethnopharmacology (Impact Factor: 3). 12/2003; 89(1):67-71. DOI: 10.1016/S0378-8741(03)00234-4
Source: PubMed


Extracts obtained from the leaves of Lavandula angustifolia Mill. (Lamiaceae) are used in Iranian folk medicine as remedies for the treatment of various inflammatory diseases. For evaluation of its probable analgesic and anti-inflammatory effects, hydroalcoholic extract, polyphenolic fraction and essential oil of the leaves of the herb were prepared and their analgesic effects were studied in mice using formalin and acetic acid-induced writhing tests. Carrageenan test in rats was used for assessment of anti-inflammatory activity of above-mentioned fractions. Results showed that while the hydroalcoholic extract (400-1600 mg/kg, p.o.) inhibited only the second phase of formalin test, the polyphenolic fraction (800 and 1600 mg/kg, p.o.) and essential oil (100 and 200 mg/kg, p.o.) suppressed both phases. In acetic acid-induced writhing test, polyphenolic fraction (400 and 800 mg/kg, p.o.) and essential oil (100 and 200 mg/kg, p.o.) reduced the number of abdominal constrictions. Essential oil at a dose of 200mg/kg also inhibited carrageenan-induced paw edema. Results of the present study confirm the traditional use of Lavandula angustifolia for the treatment of painful and inflammatory conditions and calls for further investigations to determine the active chemical constituent(s).

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Available from: Alireza Ghannadi, Feb 09, 2014
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    • "In India, it has been successfully introduced for its cultivation in temperate regions of Kashmir Valley (Tajjudin et al. 1983; Shawl and Kumar 2000). In addition to its perfumery value, lavender oil is also used in aromatherapy to relieve depression, insomnia, migraine headache, nerve sprains and joint pains (Cavanagh and Wilkinson 2002; Hajhashemi et al. 2003; Sasannejad et al. 2012). Lavandula species are conventionally propagated through seeds or stem cuttings. "
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    ABSTRACT: A protocol for long-term in vitro conservation of multiple shoot cultures of Lavandula officinalis and regeneration of true-to-type plants from them is described here. Multiple shoots were developed from apical bud explants on a modified vitamin-enriched Murashige and Skoog’s basal medium supplemented with 2.5 mg/l Kinetin. The cultures were subsequently conserved in vitro for 6 years under slow growth conditions imposed by lowering the sucrose level from 3 to 2.0 % and increasing the agar concentration from 0.8 to 1.2 % in this medium. Complete plant regeneration from in vitro conserved shoots was achieved through axillary shoot proliferation on original 2.5 mg/l Kinetin containing medium followed by rooting of individual shoots in a hormone-free half strength MS basal medium. The genetic fidelity of the regenerated plants was tested by random amplified polymorphic DNA analysis. Twenty one arbitrary decamer primers produced a total of 64 scorable bands (1–6 bands/primer) in the size range of 100–5,148 bp. All DNA fingerprints with these primers displayed monomorphic band profiles indicating homogeneity among the regenerated progeny and their uniformity with the donor parent. Head-space gas chromatographic analysis of the leaf tissue excised from mother as well as regenerated plants also revealed a similar qualitative and quantitative profile of volatile terpenes. The utility of the developed protocol for long-term ex situ conservation and quality plant production for cultivation of this high value aromatic herb is discussed.
    Plant Cell Tissue and Organ Culture 10/2014; 120(2). DOI:10.1007/s11240-014-0637-7 · 2.13 Impact Factor
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    • "The plant is used in traditional and folk medicines of different parts of the world for the treatment of several gastrointestinal, nervous and rheumatic disorders [29] . Lavender is comprised of over 100 constituents, among which the primary components are linalool and linalyl acetate, α-pinene, limonene,1,8-cineole,cis-andtrans- ocimene, 3-octanone, camphor, caryophyllene, terpinen-4- ol, and flavonoids [30] . "
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    ABSTRACT: Objective: To evaluate the protective effect of Lavandula officinalis (L. officinalis) extract against blood-brain barrier (BBB) permeability and its possible mechanisms in an experimental model of stroke. Methods: Focal cerebral ischemia was induced by the transient occlusion of the middle cerebral artery for 1 h in rats. Lavender extract (100, 200 mg/kg i.p.) was injected for 20 consecutive days. BBB permeability and oxidative stress biomarkers were evaluated using standard methods. Results: The results of this study showed that L. officinalis ethanolic extract significantly reduced the BBB permeability in experimental groups when compared with ischemia group. The lavender extract significantly reduced malondialdehyde levels of plasma and brain tissue in intact group when compared with control group. Conclusions: L. officinalis extract reduced blood brain barrier permeability and alleviated neurological function in rats, and the mechanism may be related to augmentation in endogenous antioxidant defense and inhibition of oxidative stress in the rat brain.
    Asian Pacific Journal of Tropical Medicine 09/2014; 7S1:S421-6. DOI:10.1016/S1995-7645(14)60269-8 · 0.93 Impact Factor
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    • "They are highly complex volatile compounds which consist of about twenty to sixty components in various concentrations. It has been long acknowledged that the essential oil of Lavandula angustifolia exhibits various biological activities including antimicrobial, antimutagenic, anti-inflammatory and analgesic properties (Hajhashemi et al. 2003; D'auria et al. 2005; Evandri et al. 2005; Fabio et al. 2007). "
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    ABSTRACT: The aim of this study was to investigate the mode of action of the lavender essential oil (LV) on antimicrobial activity against multi-drug-resistant Escherichia coli J53 R1 when used singly and in combination with piperacillin. In the time-kill analysis, a complete killing of bacteria was observed based on colony counts within 4 h when LV was combined with piperacillin during exposure at determined FIC concentrations. Analysis of the membrane permeabilizing effects of LV on treated cultures through their stability against sodium dodecyl sulphate revealed that the LV played a role in disrupting the bacterial cell membrane. The finding is further supported by scanning electron microscopy analysis and zeta potential measurement. In addition, reduction in light production expression of E. coli [pSB1075] by the LV showed the presence of potential quorum sensing (QS) inhibitors. These results indicated that the LV has the potential to reverse bacterial resistance to piperacillin in E. coli J53 R1. It may operate via two mechanisms: alteration of outer membrane permeability and inhibition of bacterial QS. These findings offer a novel approach to develop a new option of phytopharmaceuticals against multi-drug-resistant E. coli.
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