Article

Swine Workers and Swine Influenza Virus Infections

University of Iowa College of Public Health, Iowa City, Iowa, USA.
Emerging infectious diseases (Impact Factor: 7.33). 01/2008; 13(12):1871-8. DOI: 10.3201/eid1312.061323
Source: PubMed

ABSTRACT In 2004, 803 rural Iowans from the Agricultural Health Study were enrolled in a 2-year prospective study of zoonotic influenza transmission. Demographic and occupational exposure data from enrollment, 12-month, and 24-month follow-up encounters were examined for association with evidence of previous and incident influenza virus infections. When proportional odds modeling with multivariable adjustment was used, upon enrollment, swine-exposed participants (odds ratio [OR] 54.9, 95% confidence interval [CI] 13.0-232.6) and their nonswine-exposed spouses (OR 28.2, 95% CI 6.1-130.1) were found to have an increased odds of elevated antibody level to swine influenza (H1N1) virus compared with 79 nonexposed University of Iowa personnel. Further evidence of occupational swine influenza virus infections was observed through self-reported influenza-like illness data, comparisons of enrollment and follow-up serum samples, and the isolation of a reassortant swine influenza (H1N1) virus from an ill swine farmer. Study data suggest that swine workers and their nonswine-exposed spouses are at increased risk of zoonotic influenza virus infections.

Full-text

Available from: Troy Mccarthy, Jun 12, 2015
0 Followers
 · 
132 Views
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: The paper describes an outbreak of swine influenza, which was the first H1N2 outbreak confirmed in pig farm in Poland. The outbreak occurred in October 2011 in the farrows to finish farm, first in the fattening units, and subsequently in the reproduction and weaning sectors. Samples of the lungs taken from dead gilts were tested by the use of real time and multiplex PCR, sequencing, and virus isolation methods. The amplification of the genetic material extracted from the lungs confirmed the presence of the M1 gene sequence of type A influenza virus. Using multiplex PCR, the bands of 241 bp, typical for HA1 human-like type, and of 791 bp, typical for NA2, were demonstrated. The new isolates of swine influenza virus, named A/swine/Poland/15817/2011, were obtained in embryonated SPF chicken eggs and MDCK cells. Phylogenetic analysis of the HA and NA genes of the isolate revealed that the virus is of human swine linkage closely related to European human-like viruses. On the basis of such results, it can be stated that A/Swine/Poland/15817/2011 is a new swine influenza virus, representing the H1N2 subtype strain, which emerged in Poland.
    Bulletin- Veterinary Institute in Pulawy 01/2013; 57(1). DOI:10.2478/bvip-2013-0002 · 0.37 Impact Factor
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Swine influenza virus is endemic in the United States swine population. Vaccination of swine against influenza is the most reliable method for decreasing influenza infection in swine. Therefore, new swine influenza vaccines that can be produced rapidly to respond to emerging subtypes or clusters are needed. In these studies the alphavirus replicon technology was utilized to produce several different swine influenza vaccine candidates. A recombinant hemagglutinin (HA) protein vaccine protected against homologous pandemic H1N1 challenge at several different doses. Replicon particle (RP) vaccines expressing the HA proteins of pandemic H1N1 and an H3N2 virus also protected against subsequent homologous challenge. Further, an RP vaccine expressing the conserved nucleoprotein (NP) of an H3N2 provided a level of protection against heterologous pandemic H1N1 challenge. In addition to evaluating the efficacy, these studies also demonstrate the safety of swine influenza RP vaccines. The HA RP vaccine was not shed or spread from vaccinated animals and no reversion to virulence was detected following vaccination of both host and non-host species. Thus, these studies demonstrate that swine influenza RP vaccines are both safe and efficacious.
  • Source