Article

Evaluation of the prebiotic properties of wheat arabinoxylan fractions and induction of hydrolase activity in gut microflora.

Institute of Food Research, Norwich Research Park, Colney, NR4 7UA, Norwich, UK.
International Journal of Food Microbiology (impact factor: 3.33). 04/2008; 123(1-2):166-70. DOI:10.1016/j.ijfoodmicro.2007.11.007 pp.166-70
Source: PubMed

ABSTRACT Dietary supplementation with prebiotics may result in the stimulation of the growth of beneficial bacteria such as lactobacilli and bifidobacteria in the human gastrointestinal tract. The effect of water-unextractable arabinoxylans (WU-AX) derived from wheat on the modulation of gut bacterial composition was investigated using a mixed culture fermentation system. A prebiotic index (PI) score of 2.03 was obtained after addition of 1% (w/v) WU-AX to a pH-controlled stirred anaerobic fermentation vessel. Pretreatment of the WU-AX with endo-beta-1,4-xylanase resulted in significantly higher PI value (3.48) indicating that pretreatment provided oligomers that were better utilised by the gut bacteria. The extracellular hydrolytic enzymes xylanase and ferulic acid esterase are both required for bacterial metabolism of WU-AX and both activities were present in supernatants derived from the mixed batch cultures. Addition of the WU-AX substrates to the batch cultures produced several fold increases of bacterial synthesis of both enzymes, and these increases were greater when the WU-AX substrate was pretreated with xylanase.

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Keywords

anaerobic fermentation vessel
 
bacterial metabolism
 
bacterial synthesis
 
batch cultures
 
beneficial bacteria
 
Dietary supplementation
 
endo-beta-1,4-xylanase
 
enzymes
 
extracellular hydrolytic enzymes xylanase
 
ferulic acid esterase
 
gut bacteria
 
gut bacterial composition
 
higher PI value
 
human gastrointestinal tract
 
lactobacilli
 
mixed batch cultures
 
mixed culture fermentation system
 
modulation
 
prebiotic index
 
Pretreatment