Design of cationic microspheres based on aminated gelatin for controlled release of peptide and protein drugs.

School of Pharmacy, Hokkaido Phamaceutical University, Hokkaido, Japan.
Drug Delivery (Impact Factor: 2.02). 02/2008; 15(2):113-7. DOI: 10.1080/10717540801905124
Source: PubMed

ABSTRACT Two different types of cationized microspheres based on a native cationic gelatin (NGMS) and aminated gelatin with ethylendiamine (CGMS) were investigated for the controlled release of three model acidic peptide/protein drugs with different molecular weights (MWs) and isoelectric points (IEPs). Recombinant human (rh)-insulin (MW: 5.8 kDa, IEP: 5.3), bovine milk lactoalbumin, BMLA (MW: 14 kDa, IEP: 4.3), and bovine serum albumin (BSA MW: 67 kDa, IEP: 4.9) were used as model acidic peptide/protein drugs. The in vitro release profiles of these acidic peptide/protein drugs from NGMS and CGMS were compared and different periods of cross-linking were obtained. The slower release of these acidic peptide/protein drugs from CGMS compared with those from NGMS with cross-linking for 48 hr. was caused by the suppression of burst release during the initial phase. The degree of suppression of burst release of the three peptide/protein drugs during the initial phase by CGMS was in the following order: (rh)-insulin > BMLA > BSA. The release of insulin with a lower molecular weight from CGMS was particularly suppressed compared with the other two drugs with higher molecular weights in the initial phase. The control of the release rate of acidic peptide/protein drugs from gelatin microsphere can be achieved by amination of gelatin. Therefore, CGMS is useful for the controlled release of acidic peptide/ protein drugs.

  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: Gene therapy is a widespread and promising treatment of many diseases resulting from genetic disorders, infections and cancer. The feasibility of the gene therapy is mainly depends on the development of appropriate method and suitable vectors. For an efficient gene delivery, it is very important to use a carrier that is easy to produce, stable, non-oncogenic and non-immunogenic. Currently most of the vectors actually suffer from many problems. Therefore, the ideal gene therapy delivery system should be developed that can be easily used for highly efficient delivery and able to maintain long-term gene expression, and can be applicable to basic research as well as clinical settings. This article provides a brief over view on the concept and aim of gene delivery, the different gene delivery systems and use of different materials as a carrier in the area of gene therapy.
    Advanced Materials Research 07/2014; 995:29-47.
  • Source
    [Show abstract] [Hide abstract]
    ABSTRACT: This study demonstrates a facile but effective method to construct glucose coated magnetic Fe3O4 nanoparticles for drug delivery through a glycothermal method (100–200 °C). In this process, magnetic Fe3O4 nanoparticles were firstly prepared via a glycothermal reaction of Fe (III) salts (e.g., FeCl3·6H2O) in ethylene glycol. Second, the polymerized glucose was used as a carbon layer to coat on the surface of Fe3O4 nanoparticles. The polymerized glucose layer is biocompatible and can provide functional –OH groups for interaction with biomolecules that contain –COOH, –SH, and –NH2 groups. The particle characteristics (morphology, size, surface property) were identified by various techniques such as transmission electron microscopy (TEM), Fourier transform infrared (FT-IR) spectroscopy, and ultraviolet visible (UV–vis) spectrum. Finally, the polymerized-glucose coated magnetic Fe3O4 nanocomposites were used in drug delivery test at ambient conditions, and the loading or release performance of aspirin was assessed by using UV–vis spectroscopy. The influence of pH and ionic strength on the adsorption capacity has also been investigated. The findings in this study will be useful for engineering iron oxide nanoparticles for potential drug delivery applications.
    Powder Technology. 236:157–163.
  • [Show abstract] [Hide abstract]
    ABSTRACT: Functional polymer brushes have been utilized extensively for the immobilization of biomolecules, which is of crucial importance for the development of biosensors and biotechnology. Recent progress in polymerization methods, in particular surface-initiated atom transfer radical polymerization (ATRP), has provided a unique means for the design and synthesis of new biomolecule-functionalized polymer brushes. This current review summarizes such recent research activities. The different preparation strategies for biomolecule immobilization through polymer brush spacers are described in detail. The functional groups of the polymer brushes used for biomolecule immobilization include epoxide, carboxylic acid, hydroxyl, aldehyde, and amine groups. The recent research activities indicate that functional polymer brushes become versatile and powerful spacers for immobilization of various biomolecules to maximize their functionalities. This review also demonstrates that surface-initiated ATRP is used more frequently than other polymerization methods in the designs of new biomolecule-functionalized polymer brushes.
    Chemical Society Reviews 01/2013; · 24.89 Impact Factor