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Antimicrobial surface functionalization of plastic catheters by silver nanoparticles

AcryMed, Inc., 9560 SW Nimbus Avenue, Beaverton, OR 97008, USA.
Journal of Antimicrobial Chemotherapy (Impact Factor: 5.44). 04/2008; 61(4):869-76. DOI: 10.1093/jac/dkn034
Source: PubMed

ABSTRACT To test the antimicrobial activity and evaluate the risk of systemic toxicity of novel catheters coated with silver nanoparticles.
Catheters were coated with silver using AgNO3, a surfactant and N,N,N ',N '-tetramethylethylenediamine as a reducing agent. Particle size was determined by electron microscopy. Silver release from the catheters was determined in vitro and in vivo using radioactive silver ((110m)Ag+). Activity on microbial growth and biofilm formation was evaluated against pathogens most commonly involved in catheter-related infections, and the risk for systemic toxicity was estimated by measuring silver biodistribution in mice implanted subcutaneously with (110 m)Ag+-coated catheters.
The coating method yielded a thin ( approximately 100 nm) layer of nanoparticles of silver on the surface of the catheters. Variations in AgNO3 concentration translated into proportional changes in silver coating (from 0.1 to 30 microg/cm(2)). Sustained release of silver was demonstrated over a period of 10 days. Coated catheters showed significant in vitro antimicrobial activity and prevented biofilm formation using Escherichia coli, Enterococcus, Staphylococcus aureus, coagulase-negative staphylococci, Pseudomonas aeruginosa and Candida albicans. Approximately 15% of the coated silver eluted from the catheters in 10 days in vivo, with predominant excretion in faeces (8%), accumulation at the implantation site (3%) and no organ accumulation (< or = 0.1%).
A method to coat plastic catheters with bioactive silver nanoparticles was developed. These catheters are non-toxic and are capable of targeted and sustained release of silver at the implantation site. Because of their demonstrated antimicrobial properties, they may be useful in reducing the risk of infectious complications in patients with indwelling catheters.

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    Edited by Protima Rauwel, Erwan Rauwel, Stanislav Ferdov, Mangala P. Singh, 03/2015; Hindawi Publishing Corporation.
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    ABSTRACT: Deposition of charge-stabilized silver nanoparticles, from suspensions containing tannic acid (tannin), on mica covered by the poly(allylamine hydrochloride) (PAH) supporting layer was studied. Initially, the size distribution, stability and electrokinetic properties of the silver particles were determined using transmission electron microscopy (TEM), dynamic light scattering method (DLS) and microelectrophoretic measurements. Analogously, the physicochemical properties of tannin were thoroughly determined, especially its electrokinetic charge and the adsorption isotherm at PAH-covered mica substrate. Afterwards, the kinetics of silver particle deposition in the presence of controlled quantities of tannin was measured at ionic strength of 10−2 M. An abrupt decrease in the maximum coverage of silver particle monolayers was observed from 0.28 (in the absence of tannin) to 0.03 for 50 mg L−1 at 3 × 10−5 M of tannin in the suspension. These results were interpreted using the random sequential adsorption model (RSA), according to the competitive adsorption of tannin. Obtained results confirmed crucial influence of trace amounts of organic contaminants on the deposition of silver nanoparticles at solid substrates. Besides significance for basic science, this finding has practical consequences enabling to improve the biocidal activity of silver nanoparticles.
    Colloids and Surfaces A Physicochemical and Engineering Aspects 04/2015; 477:70–76. DOI:10.1016/j.colsurfa.2015.03.019 · 2.35 Impact Factor

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