Population Evidence of Cryptic Species and Geographical Structure in the Cosmopolitan Ectomycorrhizal Fungus, Tricholoma scalpturatum

Laboratoire Evolution et Diversité Biologique, UMR 5174 CNRS-UPS-ENFA, Bât. 4R3, Université Paul Sabatier-Toulouse III, 118 route de Narbonne, 31062, Toulouse Cedex 9, France.
Microbial Ecology (Impact Factor: 2.97). 03/2008; 56(3):513-24. DOI: 10.1007/s00248-008-9370-2
Source: PubMed


Tricholoma scalpturatum is an ectomycorrhizal fungus that forms symbioses with roots of diverse trees and shrubs. It is commonly encountered in a wide range of habitats, across temperate ecosystems. A previous study has revealed a high genetic diversity at a local scale, and ruderal abilities. To examine genetic structure at a large geographical scale, a total of 164 basidiocarps were collected from 30 populations located in Western Europe, from Spain to Scandinavia. These samples were analyzed by three molecular methods with different levels of resolution: inter-simple sequence repeats (ISSRs), restriction fragment length polymorphisms (RFLPs) in the rDNA internal transcribed spacer (ITS), and ITS sequence analysis. Considerable genetic variation was found, and the morphospecies was separated into two genetic groups that were distinct from each other. The ISSR data and the relatively low percentage value (96%) of shared sequence polymorphisms in the ITS between isolates from the two groups, strongly suggest cryptic species and long-lasting separation. No geographical exclusion was detected for these two widely distributed taxa. However, high estimates of population differentiation were observed in each group, including between populations less than a few kilometers apart. This result provides evidence for limited gene flow and/or founding effects. It also indicates that T. scalpturatum does not constitute a random mating population, and the hypothesis of endemism cannot be excluded for this cosmopolitan wind-dispersed fungus.

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Available from: Jacob Heilmann-Clausen, Dec 19, 2013
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    • "In the case of microorganisms, it has been said that microbial taxa will not exhibit endemism because their enormous populations remove dispersal as an effective constraint on geographical range [25]. The results from our research support previous studies with other microorganisms, including fungi, that there are very few truly cosmopolitan species [2], [17], [18]. Because T. discophora is a species complex that includes species with restricted geography and ecology, we hypothesize that the limited effective dispersal mechanisms over long geographic distances is affecting their distribution. "
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