Article

Comprehensive evaluation of the estrogen receptor alpha gene reveals further evidence for association with type 2 diabetes enriched for nephropathy in an African American population.

Center for Human Genomics, Wake Forest University School of Medicine, Winston-Salem, NC, USA.
Human Genetics (Impact Factor: 4.52). 05/2008; 123(4):333-41. DOI: 10.1007/s00439-008-0482-z
Source: PubMed

ABSTRACT We previously investigated the estrogen receptor alpha gene (ESR1) as a positional candidate for type 2 diabetes (T2DM), and found evidence for association between the intron 1-intron 2 region of this gene and T2DM and/or nephropathy in an African American (AA) population. Our objective was to comprehensively evaluate variants across the entire ESR1 gene for association in AA with T2DM and end stage renal disease (T2DM-ESRD). One hundred fifty SNPs in ESR1, spanning 476 kb, were genotyped in 577 AA individuals with T2DM-ESRD and 596 AA controls. Genotypic association tests for dominant, additive, and recessive models, and haplotypic association, were calculated using a chi(2) statistic and corresponding P value. Thirty-one SNPs showed nominal evidence for association (P < 0.05) with T2DM-ESRD in one or more genotypic model. After correcting for multiple tests, promoter SNP rs11964281 (nominal P = 0.000291, adjusted P = 0.0289), and intron 4 SNPs rs1569788 (nominal P = 0.000754, adjusted P = 0.0278) and rs9340969 (nominal P = 0.00109, adjusted P = 0.0467) remained significant at experimentwise error rate (EER) P </= 0.05 for the dominant class of tests. Twenty-three of the thirty-one associated SNPs cluster within the intron 4-intron 6 regions. Gender stratification revealed nominal evidence for association with 35 SNPs in females (352 cases; 306 controls) and seven SNPs in males (225 cases; 290 controls). We have identified a novel region of the ESR1 gene that may contain important functional polymorphisms in relation to susceptibility to T2DM and/or diabetic nephropathy.

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    • "Also, ESR1, the estrogen receptor 1 gene, was shown associated with HDL-C response to atenolol. Variants in ESR1 have been associated with many traits including type 2 diabetes in African Americans [34], and HDL-C response to atorvastatin in women [29]. The last gene of particular interest is LIPC, hepatic lipase, which is involved in lipoprotein metabolism, and other SNPs in the gene have also been associated with HDL-C in GWAS [11]. "
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    • "Estrogen has shown to have antidiabetic actions in both rodent models as well as clinical trials (Louet et al. 2004; Margolis et al. 2004; Seed 2002) . Our association results suggest a differential association with ESR1 variants and T2DM-ESRD in males (7 SNPs) versus females (35 SNPs) (Keene et al 2008b). "
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