Comprehensive spatiotemporal transcriptomic analyses of the ganglionic eminences demonstrate the uniqueness of its caudal subdivision

School of Life Sciences, Ecole Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.
Molecular and Cellular Neuroscience (Impact Factor: 3.84). 05/2008; 37(4):845-56. DOI: 10.1016/j.mcn.2008.01.009
Source: PubMed


The elucidation of mechanisms underlying telencephalic neural development has been limited by the lack of knowledge regarding the molecular and cellular aspects of the ganglionic eminence (GE), an embryonic structure that supplies the brain with diverse sets of GABAergic neurons. Here, we report a comprehensive transcriptomic analysis of this structure including its medial (MGE), lateral (LGE) and caudal (CGE) subdivisions and its temporal dynamics in 12.5 to 16 day-old rat embryos. Surprisingly, comparison across subdivisions showed that CGE gene expression was the most unique providing unbiased genetic evidence for its differentiation from MGE and LGE. The molecular signature of the CGE comprised a large set of genes, including Rwdd3, Cyp26b1, Nr2f2, Egr3, Cpta1, Slit3, and Hod, of which several encode cell signaling and migration molecules such as WNT5A, DOCK9, VSNL1 and PRG1. Temporal analysis of the MGE revealed differential expression of unique sets of cell specification and migration genes, with early expression of Hes1, Lhx2, Ctgf and Mdk, and late enrichment of Olfm3, SerpinE2 and Wdr44. These GE profiles reveal new candidate regulators of spatiotemporally governed GABAergic neuronogenesis.

13 Reads
  • Source
    • "Gsx2 (also called Gsh2) is enriched in (but not restricted to) the LGE and CGE from early development (Figure 1) and has been directly implicated in promoting the CR+ interneuron identity (Xu et al., 2010a). The orphan nuclear receptor COUP-TFII shows restricted expression in the CGE (Kanatani et al., 2008; Willi-Monnerat et al., 2008) and, together with COUP-TFI, is required for the caudal migration of cortical interneurons (Tripodi et al., 2004). Moreover, in Nkx2.1 mutant mice, a higher number of CR+ and VIP+ cortical interneurons are generated and COUP-TFII is ectopically expressed in the MGE (Butt et al., 2008). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Cortical calretinin (CR)-expressing interneurons represent a heterogeneous subpopulation of about 10-30% of GABAergic interneurons, which altogether total ca. 12-20% of all cortical neurons. In the rodent neocortex, CR cells display different somatodendritic morphologies ranging from bipolar to multipolar but the bipolar cells and their variations dominate. They are also diverse at the molecular level as they were shown to express numerous neuropeptides in different combinations including vasoactive intestinal polypeptide (VIP), cholecystokinin (CCK), neurokinin B (NKB) corticotrophin releasing factor (CRF), enkephalin (Enk) but also neuropeptide Y (NPY) and somatostatin (SOM) to a lesser extent. CR-expressing interneurons exhibit different firing behaviors such as adapting, bursting or irregular. They mainly originate from the caudal ganglionic eminence (CGE) but a subpopulation also derives from the dorsal part of the medial ganglionic eminence (MGE). Cortical GABAergic CR-expressing interneurons can be divided in two main populations: VIP-bipolar interneurons deriving from the CGE and SOM-Martinotti-like interneurons originating in the dorsal MGE. Although bipolar cells account for the majority of CR-expressing interneurons, the roles they play in cortical neuronal circuits and in the more general metabolic physiology of the brain remained elusive and enigmatic. The aim of this review is, firstly, to provide a comprehensive view of the morphological, molecular and electrophysiological features defining this cell type. We will, secondly, also summarize what is known about their place in the cortical circuit, their modulation by subcortical afferents and the functional roles they might play in neuronal processing and energy metabolism.
    Frontiers in Neuroanatomy 06/2014; 8:52. DOI:10.3389/fnana.2014.00052 · 3.54 Impact Factor
  • Source
    • "For example Gsx2, Dlx2, and Mash1 are three transcription factors involved in neurogenesis, patterning and migration and are expressed throughout the ganglionic eminences. Despite this similarity, there are genetic differences and precise expression domains that are starting to be described (Flames et al., 2007; Willi-Monnerat et al., 2008). Most importantly, the expression of Nkx2.1, a Shh responsive gene (Xu et al., 2010), defines the MGE and the ventral CGE, discerning these tissues from the LGE and the dorsal CGE (Sussel et al., 1999). "
    [Show abstract] [Hide abstract]
    ABSTRACT: Cortical interneurons represent 20% of the cells in the cortex. These cells are local inhibitory neurons whose function is to modulate the firing activities of the excitatory projection neurons. Cortical interneuron dysfunction is believed to lead to runaway excitation underlying (or implicated in) seizure-based diseases, such as epilepsy, autism, and schizophrenia. The complex development of this cell type and the intricacies involved in defining the relative subtypes are being increasingly well defined. This has led to exciting experimental cell therapy in model organisms, whereby fetal-derived interneuron precursors can reverse seizure severity and reduce mortality in adult epileptic rodents. These proof-of-principle studies raise hope for potential interneuron-based transplantation therapies for treating epilepsy. On the other hand, cortical neurons generated from patient iPSCs serve as a valuable tool to explore genetic influences of interneuron development and function. This is a fundamental step in enhancing our understanding of the molecular basis of neuropsychiatric illnesses and the development of targeted treatments. Protocols are currently being developed for inducing cortical interneuron subtypes from mouse and human pluripotent stem cells. This review sets out to summarize the progress made in cortical interneuron development, fetal tissue transplantation and the recent advance in stem cell differentiation toward interneurons.
    Frontiers in Cellular Neuroscience 03/2013; 7:10. DOI:10.3389/fncel.2013.00010 · 4.29 Impact Factor
  • Source
    • "Understanding the hierarchies of transcriptional circuitry for each neuronal subtype will be key to determining which transcription factors and regulatory RNAs will most successfully effect the desired reprogramming events for a given subtype. Genomic expression profiling efforts are making progress in this direction (Arlotta et al., 2005; Molyneaux et al., 2009; Nelson et al., 2006; Willi-Monnerat et al., 2008). "
    [Show abstract] [Hide abstract]
    ABSTRACT: The human cerebral cortex is an immensely complex structure that subserves critical functions that can be disrupted in developmental and degenerative disorders. Recent innovations in cellular reprogramming and differentiation techniques have provided new ways to study the cellular components of the cerebral cortex. Here, we discuss approaches to generate specific subtypes of excitatory cortical neurons from pluripotent stem cells. We review spatial and temporal aspects of cortical neuron specification that can guide efforts to produce excitatory neuron subtypes with increased resolution. Finally, we discuss distinguishing features of human cortical development and their translational ramifications for cortical stem cell technologies.
    Neuron 05/2011; 70(4):645-60. DOI:10.1016/j.neuron.2011.05.006 · 15.05 Impact Factor
Show more

Similar Publications