An aerobic, rod-shaped, Gram-positive, oxidase-negative, catalase-positive bacterial isolate, strain FCC-01(T), originating as a contaminant of hairspray was characterized using phenotypic and molecular taxonomic methods. A 16S rRNA gene sequence analysis revealed that the isolate belonged to the genus Microbacterium and represented an evolutionary lineage that was distinct from recognized Microbacterium species. Cell-wall hydrolysate from the isolate contained ornithine and the cell-wall sugars consisted of rhamnose and galactose. The main respiratory quinones were MK-12 (38 %) and MK-11 (35 %). The major cellular fatty acids were anteiso-C(15 : 0) (48 %), anteiso-C(17 : 0) (35 %) and iso-C(16 : 0) (11 %). The DNA G+C content was 69 mol%. The isolate showed <98 % 16S rRNA gene sequence similarity with respect to all of the Microbacterium species with validly published names. On the basis of the morphological, physiological and chemotaxonomic data and the results of the comparative 16S rRNA gene sequence analysis, this isolate represents a novel species of the genus Microbacterium, for which the name Microbacterium hatanonis sp. nov. is proposed. The type strain is FCC-01(T) (=JCM 14558(T) =DSM 19179(T)).
"Members of the genus Microbacterium can be isolated from a wide range of different environmental habitats from soil to insects to human clinical specimens to marine environments (Evtushenko & Takeuchi, 2006; Park et al., 2008; Bakir et al., 2008; Takeuchi & Hatano, 1998a, b; Lee et al., 2006; Shivaji et al., 2007; Collins & Bradbury, 1992) and plants, having been reported from the phyllosphere of sugar beet and spring wheat, and as endophytes in sweet corn and cotton (Thompson et al., 1993; Legard et al., 1994; McInroy & Kloepper, 1995). The genus Microbacterium , established by Orla-Jensen (1919) and emended by Collins et al. (1983), comprises a diverse collection of Gram-positive, non-spore-forming, rod-shaped bacteria. "
[Show abstract][Hide abstract] ABSTRACT: Microbacterium strain AI-S262(T) was isolated from the rhizoplane of neem seedlings in the Botanical garden of Tamilnadu Agricultural University, Coimbatore, India, and subjected to phenotypic, chemotaxonomic and genetic characterization. Cells of this strain were Gram-stain-positive, motile, non-spore-forming, short rods and formed light-yellow-pigmented colonies on nutrient agar. Strain AI-S262(T) contained MK-12 and MK-13 as the main respiratory quinones, anteiso-C(15 : 0), anteiso-C(17 : 0) and iso-C(16 : 0) as the predominant fatty acids, peptidoglycan-type B2beta with glycolyl residues, and had a DNA G+C content of 69.5 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed 98.0-98.6 % pair-wise similarity with respect to close relatives in the genus Microbacterium. DNA-DNA hybridization experiments revealed a low level of DNA-DNA relatedness (less than 39%) between strain AI-S262(T) and its closest relatives. Data from DNA-DNA hybridization and phenotypic analyses supported the conclusion that strain AI-S262(T) represents a novel species in the genus Microbacterium, for which the name Microbacterium azadirachtae sp. nov. is proposed. The type strain is AI-S262(T) (=JCM 15681(T) =LMG 24772(T) =KCTC 19668(T)).
International Journal of Systematic and Evolutionary Microbiology 10/2009; 60(Pt 7):1687-92. DOI:10.1099/ijs.0.015800-0 · 2.51 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: Strains JS54-2(T) and JS63-1 were isolated from seawater at Jeju, Korea. Cells of these strains were Gram-positive, non-motile, non-spore-forming, short rods and formed yellow-pigmented colonies on tryptic soy agar. The strains had chemotaxonomic markers that were consistent with their classification in the genus Microbacterium, i.e. MK-11, MK-12 and MK-10 as the major menaquinones, fatty acids that were predominantly iso- and anteiso-branched, galactose and glucose as the cell-wall sugars, peptidoglycan-type B2beta with glycolyl residues and DNA G+C contents of 69.3-69.6 mol%. A phylogenetic analysis based on 16S rRNA gene sequences showed that the strains were closely related to Microbacterium oleivorans DSM 16091(T) and Microbacterium halophilum IFO 16062(T) (with 97.7 and 97.4 % sequence similarity, respectively) and formed a separate lineage with M. halophilum in the genus Microbacterium. Data from DNA-DNA hybridization and phenotypic analyses supported the conclusion that strains JS54-2(T) and JS63-1 represent a novel species in the genus Microbacterium, for which the name Microbacterium aquimaris sp. nov. is proposed. The type strain is JS54-2(T) (=KCTC 19124(T) =DSM 19713(T)).
International Journal of Systematic and Evolutionary Microbiology 08/2008; 58(Pt 7):1616-20. DOI:10.1099/ijs.0.65763-0 · 2.51 Impact Factor
[Show abstract][Hide abstract] ABSTRACT: A Gram-positive, non-motile, rod- or coccoid-shaped Microbacterium-like bacterium, designated strain DS-66(T), was isolated from soil of Dokdo, Korea, and its exact taxonomic position was investigated by using a polyphasic approach. Strain DS-66(T) grew optimally at 30 degrees C and pH 6.5-7.0 in the presence of 0.5-1.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain DS-66(T) belonged to the genus Microbacterium. Strain DS-66(T) had a peptidoglycan type based on B2beta with partial substitution of glutamic acid by 3-hydroxy glutamic acid (Glu/Hyg-Gly-d-Orn), and galactose, rhamnose and ribose as whole-cell sugars. The acyl type was glycolyl. Strain DS-66(T) contained MK-13, MK-12 and MK-14 as predominant menaquinones and anteiso-C(15 : 0), anteiso-C(17 : 0), iso-C(17 : 0) and iso-C(16 : 0) as major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, an unidentified phospholipid and an unidentified glycolipid. The DNA G+C content was 69.9 mol%. Phylogenetic distinctiveness, DNA-DNA relatedness data and differential phenotypic properties demonstrated that strain DS-66(T) is distinguishable from recognized Microbacterium species. On the basis of the data presented, strain DS-66(T) is considered to represent a novel species of the genus Microbacterium, for which the name Microbacterium insulae sp. nov. is proposed. The type strain is DS-66(T) (=KCTC 19247(T)=CCUG 54523(T)).
International Journal of Systematic and Evolutionary Microbiology 07/2009; 59(Pt 7):1738-42. DOI:10.1099/ijs.0.007591-0 · 2.51 Impact Factor
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