Prevalence and geographical distribution of Escherichia coli O157 in India: a 10-year survey

National Salmonella and Escherichia Centre, Central Research Institute, Kasauli (H.P.) 173204, India.
Transactions of the Royal Society of Tropical Medicine and Hygiene (Impact Factor: 1.84). 05/2008; 102(4):380-3. DOI: 10.1016/j.trstmh.2008.01.015
Source: PubMed


Escherichia coli colonizes the human gastrointestinal tract and produces a variety of diseases. Escherichia coli O157 is one of the most important pathogenic strains reported from food-borne illnesses leading to enterohemorrhagic colitis. The National Salmonella and Escherichia Centre is a national reference centre for Salmonella and Escherichia for India; it receives samples from research laboratories, hospitals and institutions for serological identification. The present study is an epidemiological survey of E. coli O157 in different regions of India. The data are based on samples received from humans, food items, animals and the environment. A total of 17 093 isolates cultured from samples were received during the 10-year period of which 5678 were from human sources. Thirty (0.5%) human samples were positive for E. coli O157. A significantly high percentage of E. coli O157 were isolated from meat (0.9%, 13/1376), milk and milk products (1.8%, 10/553), seafood (8.4%, 16/190) and water (1.6%, 8/486). The isolates were found to be distributed among domestic and wild animals, and the maximum number of isolates of E. coli O157 was detected in samples received from coastal belt areas. Escherichia coli O157 is widely distributed among humans and animals, food and environment in different geographical regions of India.

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    • "In our study, E. coli O157:H7 was detected in 2.5% of raw milk samples and 1.1% of cheese samples. In India, E. coli O157 were isolated from 1.8% of milk and milk product samples (Sehgal et al., 2008). In Ireland, E. coli O157:H7 was detected in 3.1% of Irish dairy farm herds and their milk (Lynch et al., 2012). "
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    ABSTRACT: Shiga toxin-producing Escherichia coli (STEC) 0157:1-17 is an important food-borne pathogen that has been implicated in numerous disease outbreaks worldwide. Little is known about the extent and molecular basis of antimicrobial resistance in STEC 0157:H7 of food origin. Therefore, the current study aimed to characterize the genetic basis of multidrug resistance in 54 STEC 0157:H7 strains isolated from 1600 food samples (800 meat products and 800 dairy products) collected from different street venders, butchers, retail markets, and slaughterhouses in Egypt. Thirty-one of 54(57.4%) isolates showed multidrug resistance phenotypes to at least three classes of antimicrobials. The highest incidence of antimicrobial resistance was to kanamycin (96.8%), followed by spectinomycin (93.6%), ampicillin (903%), streptomycin (87.1%), and tetracycline (80.6%). PCR and DNA sequencing were used to screen and characterize integrons and antibiotic resistance genes, and 29.6% and 5.6% of isolates were positive for class 1 and class 2 integrons, respectively. p-Lactamase-encoding genes were identified in 63.0% of isolates as follows: blarEm4 and blaTEm_52 in 35.2% and 1.9% isolates respectively; b/acmy-2 in 13.0% isolates; blacix_m in 5.6% isolates; blasHv_12 in 5.6% isolates; and bia0xA..1 in 1.9% isolate. The plasmid-mediated quinolone resistance genes were identified in 13.0% of isolates as follows: qnrB, qnrS, and aac(60-1b-cr in 5.6%, 3.7%, and 3.7% isolates, respectively. Finally, the florfenicol resistance genefloR was identified in 7.4% of isolates. This study demonstrated that meat and dairy products are potential sources of multidrug resistant STEC 0157: H7. To our knowledge, this is the first report of the occurrence of class 2 integrons, prB, qnrS, and aac(694b-cr in STEC 0157:H7. 2014 Elsevier B.V. All rights reserved.
    International Journal of Food Microbiology 01/2015; 193. DOI:10.1016/j.ijfoodmicro.2014.10.014 · 3.08 Impact Factor
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    • "Drinking water and recreational water have been implicated in the transmission of pathogens, and it was opined that the source of contamination could be either sewage or infected animals (Muniesa et al., 2006; Sehgal et al., 2008). A number of bacteria species, including coliforms and Listeria can be present in the Intestines of some humans and animals, including birds without causing infection (Ramaswany et al., 2007). "
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    ABSTRACT: Untreated abattoir effluent constitutes a reservoir for the spread of intestinal pathogens and Listeria species (though rarely considered), is one of such organisms. This study was therefore conducted to determine the status of these bacteria and others in abattoir effluent, in Lagos, Nigeria. Thirty samples of abattoir effluent were collected over a period of 6 weeks at the government central abattoir in Lagos, Nigeria. Each sample was serially diluted and pour-plated on Nutrient Agar, MacConkey Agar and Listeria Selective Agar. Mesophilic aerobic counts were enumerated. Isolated bacterial colonies were identified by standard methods and antimicrobial susceptibility test conducted using the disk diffusion technique. Heavy loads of Listeria species, Escherichia coli, Klebsiella, sp., Enterococcus faecalis, and Pseudomonas, aeruginosa, were isolated from all the samples. The antibiotic susceptibility pattern of these bacterial organisms revealed marked resistance to most of the antimicrobial agents tested. With the exception of Pseudomonas, there was no statistically significant difference between the antimicrobial resistance rate of Listeria and other bacteria isolates (P >0.05). The public health significance of these findings, particularly the abattoir effluent bacteria potential capability of transferring disease and antibiotic resistance to man, as well as the challenges posed to disease treatment was highlighted.
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    • "According to the result of serotyping, O4 (12%) was predominant, followed by O5 (8%), O60 (8%), R (6.6%), O41 (5.3%), O59, O1 (4%), O22, O21, O102, O103, O116, O69, O91 (2.6%), and O157, O34, O35, O37, O20, O141, O103, O104, O49, O148, O64, O153 (1.3%). When an analysis of the geographic distribution of E. coli O157 was done by Sehgal et al. [16], it was observed to be widely distributed in all parts of India showing wide prevalence of this strain in almost all regions of the country and 1.1% of O157 isolates were from Kerala. Though food- and water-borne diarrheal diseases are very common in the study area, the investigations are usually limited to characterisation of E. coli alone. "
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    ABSTRACT: This study aimed at detecting the prevalence of antibiotic-resistant serotypes of Escherichia coli in Cochin estuary, India. E. coli strains were isolated during the period January 2010-December 2011 from five different stations set at Cochin estuary. Water samples from five different stations in Cochin estuary were collected on a monthly basis for a period of two years. Isolates were serotyped, antibiogram-phenotyped for twelve antimicrobial agents, and genotyped by polymerase chain reaction for uid gene that codes for β-D-glucuronidase. These E. coli strains from Cochin estuary were tested against twelve antibiotics to determine the prevalence of multiple antibiotic resistance among them. The results revealed that more than 53.33% of the isolates were multiple antibiotic resistant. Thirteen isolates showed resistance to sulphonamides and two of them contained the sul 1 gene. Class 1 integrons were detected in two E. coli strains which were resistant to more than seven antibiotics. In the present study, O serotyping, antibiotic sensitivity, and polymerase chain reaction were employed with the purpose of establishing the present distribution of multiple antibiotic-resistant serotypes, associated with E. coli isolated from different parts of Cochin estuary.
    Interdisciplinary Perspectives on Infectious Diseases 09/2012; 2012(1):124879. DOI:10.1155/2012/124879
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