Prevalence and geographical distribution of Escherichia coli O157 in India: a 10-year survey.
ABSTRACT Escherichia coli colonizes the human gastrointestinal tract and produces a variety of diseases. Escherichia coli O157 is one of the most important pathogenic strains reported from food-borne illnesses leading to enterohemorrhagic colitis. The National Salmonella and Escherichia Centre is a national reference centre for Salmonella and Escherichia for India; it receives samples from research laboratories, hospitals and institutions for serological identification. The present study is an epidemiological survey of E. coli O157 in different regions of India. The data are based on samples received from humans, food items, animals and the environment. A total of 17 093 isolates cultured from samples were received during the 10-year period of which 5678 were from human sources. Thirty (0.5%) human samples were positive for E. coli O157. A significantly high percentage of E. coli O157 were isolated from meat (0.9%, 13/1376), milk and milk products (1.8%, 10/553), seafood (8.4%, 16/190) and water (1.6%, 8/486). The isolates were found to be distributed among domestic and wild animals, and the maximum number of isolates of E. coli O157 was detected in samples received from coastal belt areas. Escherichia coli O157 is widely distributed among humans and animals, food and environment in different geographical regions of India.
- Epidemiologic Reviews 02/1991; 13:60-98. · 9.27 Impact Factor
- [show abstract] [hide abstract]
ABSTRACT: An outbreak of gastrointestinal disease and haemolytic uraemic syndrome caused by Escherichia coli O157:H7 was investigated. The outbreak occurred in a day care centre located in northern Germany in August 1992 and involved 39 children and two adults. Furthermore, four asymptomatic infections were detected among the staff. Initial and secondary cases were reported over a 30-day interval, with cases occurring in three waves. Person-to-person contact and environmental contamination were assumed to be the main mode of transmission. The source of the outbreak has remained unknown but it is likely that primary or secondary contamination of the day care centre's kitchen, too, played a role in the spread of infections. The organisms were isolated from two open packs of deep-frozen stuffed cabbage rolls and turkey scallops in batter, and furthermore from swabs from two kitchen utensils. Of the 39 cases with diarrhoea, three developed a haemolytic uraemic syndrome; one of the latter patients died. In 8 of the cases as well as in four healthy adult employees, E. coli O157:H7 was isolated from stool samples, and in two stool culture-negative cases the presence of IgM antibody to O157 LPS indicated recent infection. The E. coli O157:H7 isolates from the cases and the kitchen were of identical phage type and yielded identical biochemical reactions. All E. coli O157:H7 isolates harboured stable slt-II genes. However, slt-I genes could only be demonstrated in the primary cultures and were lost during subcultivation. This is the largest outbreak caused by enterohaemorrhagic E. coli O157:H7 that has been documented in Germany so far. The high infectivity of the organism which was demonstrated by person-to-person transmission and propagation within certain groups of children stresses the need for strict hygienic measures and early case reporting when such infections occur in susceptible settings like day care centres, nursing homes, or hospitals.Zentralblatt für Bakteriologie: international journal of medical microbiology 12/1994; 281(4):534-43.
- [show abstract] [hide abstract]
ABSTRACT: A survey was performed to estimate the frequency of enterohemorrhagic Escherichia coli O157:H7 or O157:nonmotile (EHEC O157) in feces and on hides within groups of fed cattle from single sources (lots) presented for slaughter at meat processing plants in the Midwestern United States, as well as frequency of carcass contamination during processing from cattle within the same lots. Of 29 lots sampled, 72% had at least one EHEC O157-positive fecal sample and 38% had positive hide samples. Overall, EHEC O157 prevalence in feces and on hides was 28% (91 of 327) and 11% (38 of 355), respectively. Carcass samples were taken at three points during processing: preevisceration, postevisceration before antimicrobial intervention, and postprocessing after carcasses entered the cooler. Of 30 lots sampled, 87% had at least one EHEC O157-positive preevisceration sample, 57% of lots were positive postevisceration, and 17% had positive postprocessing samples. Prevalence of EHEC O157 in the three postprocessing samples was 43% (148 of 341), 18% (59 of 332) and 2% (6 of 330), respectively. Reduction in carcass prevalence from preevisceration to postprocessing suggests that sanitary procedures were effective within the processing plants. Fecal and hide prevalence were significantly correlated with carcass contamination (P = 0.001), indicating a role for control of EHEC O157 in live cattle.Proceedings of the National Academy of Sciences 04/2000; 97(7):2999-3003. · 9.74 Impact Factor