Article

[Research on method in avoiding the interference of poloxamer in determination of polysaccharides in thermosensitive gel].

Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials 12/2007; 30(11):1443-6. pp.1443-6
Source: PubMed

ABSTRACT To study a method in avoiding the interference of poloxamer in determination of polysaccharides in thermosensitive gel and develop an assay correspondingly.
After extracting the freeze dried gel with acetic ether, detected it in colorimetric method.
The interference of poloxamer was removed thoroughly, while the retention rate of the polysacchrarides showed to be 100.11% (n = 3). The average recovery was 102.6% (n = 6).
The method shows to be simple, maneuverable, stable and well reproducible.

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Keywords

assay correspondingly
 
colorimetric method
 
extracting
 
gel
 
maneuverable
 
polysacchrarides
 
retention rate
 
thermosensitive gel