C-reactive protein measurements in meat juice of pigs
ABSTRACT A time-resolved immunofluorometric assay was evaluated for measurement of C-reactive protein in meat juice from diaphragmatic muscle collected from slaughtered pigs. Analytical and clinical validation of the method was performed by using meat juice samples, obtained by freezing and thawing muscle pieces. The intra- and inter-assay coefficients of variation ranged from 2.2-5.8% to 7.9-14.3%, respectively. The limit of detection was 0.00038 microg/ml. The method measured the CRP concentrations in a linear manner with a good accuracy (r=0.99). CRP concentrations in serum were highly correlated with those in diaphragmatic meat juice (r=0.90; p<0.001). CRP concentrations were significantly higher in clinically affected pigs compared to non-diseased pigs. The assay described here provides a sensitive method for measuring CRP concentrations in meat juice, which can represent a suitable alternative to serum or blood samples and simplifies the process of sampling collection at slaughter.
- SourceAvailable from: Jaime Gómez-Laguna
[Show abstract] [Hide abstract]
- "Measurement of haptoglobin and C-reactive protein Hp and CRP were measured in serum, saliva and meat juice using time resolved immunofluorometric assays (TR-IFMAs) (Gutiérrez et al., 2008, 2009b). The limit of detection (LOD) for these assays was 0.52 and 0.47 ng/mL for Hp and CRP, respectively , and the coefficients of variation for intra-and inter-assay precision were <12% for both assays (Gutiérrez et al., 2008, 2009b). "
ABSTRACT: Concentrations of the acute phase proteins haptoglobin (Hp) and C-reactive protein (CRP) were measured in the serum, saliva and meat juice of pigs experimentally infected with a porcine reproductive and respiratory syndrome virus (PRRSv) field isolate. Sixteen PRRSv-free pigs were inoculated IM, killed in groups of four at 7, 14, 21 and 24 days post-inoculation (dpi), and samples of blood, saliva and diaphragmatic muscle were collected. Four non-infected controls were killed at 24 dpi. Significant differences in lung lesions were found between PRRSv-inoculated animals and controls. Changes in the concentrations of Hp and CRP in serum, saliva and meat juice samples were similar, peaking at 21 dpi. The correlations found suggest that the measurement of Hp and CRP in saliva and meat juice could serve as complementary, or possibly alternative, biomarkers of pig herd-health.The Veterinary Journal 07/2010; 185(1):83-7. DOI:10.1016/j.tvjl.2010.04.018 · 1.76 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: The objective of the present study was to develop a one-step, fast, competitive time-resolved fluorescent immunoassay to determine porcine serum amyloid A (SAA) by using species-specific reagents. The assay consisted of an all-in-one format involving only 55 min of incubation that was adapted and validated for use in 3 different specimens: serum, saliva, and meat juice. The method had overall within- and between-run coefficients of variation under 8% and 12%, respectively, and coefficients of determination higher than 0.93 for linearity under dilution analysis for all specimens. The limits of detection were 0.32 mg/l, 0.28 mg/l, and 1.74 mg/l for serum, saliva, and meat juice measurements, respectively. Upper and lower limits of quantification were determined for each sample type and resulted in wide assay ranges that allowed a precise SAA measurement in all the fluids investigated. Statistically significant differences (P = 0.0004 for serum and P < 0.0001 for the saliva and meat juice samples) in SAA levels were found when healthy (n = 20) and diseased (n = 20) pigs were compared. The obtained results indicate that this fast, sensitive, and robust assay for SAA measurement could be of use to determine health and welfare status in swine by employing alternative samples to serum.Journal of veterinary diagnostic investigation: official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc 09/2011; 23(5):902-8. DOI:10.1177/1040638711416623 · 1.35 Impact Factor
- Acute Phase Proteins as Early Non-Specific Biomarkers of Human and Veterinary Diseases, 10/2011; , ISBN: 978-953-307-873-1