Profiling of different bioactive compounds in functional drinks by high-performance liquid chromatography

Instituto de Fermentaciones Industriales (CSIC), Juan de la Cierva 3, 28006 Madrid, Spain.
Journal of Chromatography A (Impact Factor: 4.17). 05/2008; 1188(2):234-41. DOI: 10.1016/j.chroma.2008.02.054
Source: PubMed


In the present work, an HPLC method is proposed to simultaneously detect and quantify water- and fat-soluble vitamins, phenolic compounds, carotenoids and chlorophylls in a single run, by using an ultradeactivated C18 column and gradient separation using trifluoroacetic acid, water and methanol. It is shown that the HPLC method provides baseline separation of all these compounds with good resolution values in 40 min. Moreover, other figures of merit of the method show a good linear response and low detection limits for all the compounds considered in the present study. Furthermore, the usefulness of this method is demonstrated via its successful application to the analysis of different beverages from different natural origin (orange, strawberry, apple, peach pineapple, plum and blackcurrant juices, soybean milk, beers) without the need of any previous sample preparation. A good correlation is also found by comparing the total phenol content (measured by Folin-Ciocalteu method) with the sum of total phenolic compounds obtained using the proposed HPLC method. By using statistical tools, the main compounds associated with antioxidant activity of the extracts (measured by 1,1-diphenyl-2-picrylhydrazyl radical scavenging) were assessed.

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    • "The most suitable detection wavelength for simultaneous vitamin-polyphenol determination was 280 nm. Quantification of the different families of compounds was performed by building a calibration curve for each of them, as described by Mendiola and others (2008). Direct injection of pure vitamin standards was done by dissolving them in TFA 0.01% or 100% methanol depending on the polarity of the vitamin. "
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