Hepatitis B virus genotypes in southwest Iran: molecular, serological and clinical outcomes.

Gastroenterology and Hepatology Research Center, Namazi Hospital, Shiraz University of Medical Sciences, Shiraz, Iran.
World Journal of Gastroenterology (Impact Factor: 2.37). 04/2008; 14(10):1510-3.
Source: PubMed


To investigate the associations of hepatitis B virus (HBV) genotype with HBeAg and anti-HBe status, alanine aminotransferase (ALT) levels and HBV-DNA detection in different groups of HBV-infected patients in southwest Iran.
A total of 89 HBsAg-positive serum samples were collected from the same number of patients. All sera were then investigated to determine HBV DNA and serological markers. For all the polymerase chain reaction (PCR)-positive samples, biochemical, histopathological assays and genotyping were also performed.
Genotype D was the only type of HBV found in different clinical forms of acute and chronic infections. There was a high prevalence of HBeAg-negative HBV-infected patients with chronic hepatitis (52.7%). Out of 55 patients with chronic hepatitis, seven (12.7%) were diagnosed with cirrhosis. A significant association between the presence of anti-HBe antibody and an increase in ALT level, among either HBeAg-negative (P = 0.01) or HBeAg-positive (P = 0.026) patients, was demonstrated. No significant differences were observed between the clinical outcomes of HBeAg-positive and -negative individuals (P = 0.24).
Genotype D has been recognized as the only type of HBV found in different clinical forms of HBV infections, including cirrhosis, among the residents of southwest Iran. Anti-HBe possibly plays a role in disease progression in some patients with chronic hepatitis, at least for a period of disease.

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Available from: Mehrzad Banihashemi, Oct 05, 2015
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    • "In this paper, we have analyzed HCC cases from Iran, a Middle East/Central Asian country of moderate incidence of HCC (ASR of 2.1 per 100,000 person-years in both sexes) despite the relatively high prevalence of chronic carriage of HBV, mainly genotype D [16] [17] [18], in the population and the document presence of aflatoxin, at least levels, in several components of the diet [19] [20] [21]. With the objective of documenting possible association between genotype D and molecular hallmarks of HCC detected in high incidence areas, we have analyzed TP53 R249S mutations and HBV double mutations. "
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    ABSTRACT: High incidence of HCC is mostly due to the combination of two major risk factors, chronic infection with hepatitis B (HBV) and/or C (HCV) viruses and exposure to the mycotoxin aflatoxin B(1), which induces a particular mutation at codon 249 in TP53 (R249S). Eight genotypes of HBV are diversely found in high and low incidence areas. Regardless of documented strong associations between TP53 R249S mutation and HBV genotypes B, C, A or E, there is no report of such association for genotype D despite of the presence of aflatoxin in areas with high prevalence of HBV genotype D. In Iran, 3% of the population is chronically infected with HBV, predominantly genotype D. Twenty-one histologically confirmed HCC cases from Iran were analyzed for TP53 R249S and HBV double mutations 1762(T)/1764(A), hallmarks of more pathogenic forms of HBV. We did not detect any of these mutations. In addition, we report the only case identified so far carrying both R249S mutation and chronic HBV genotype D, a patient from The Gambia in West Africa. This paper suggests that association between HBV genotype D and aflatoxin-induced TP53 mutation is uncommon, explaining the relatively lower incidence of HCC in areas where genotype D is highly prevalent.
    Hepatitis research and treatment 08/2011; 2011(1):475965. DOI:10.1155/2011/475965
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    • "In this study 71.5% of volunteers referred to the behavioral counseling center of Hamadan were male which is explainable with due attention to greater high risk and digression behaviors among men. Numerous incidences of HBV, HCV and HIV infections among homosexual and bisexual men in the different parts of USA confirm increases of acquiring these viral infections.[1][2][7][8][9][10] "
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    ABSTRACT: Human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV) are three important prevalent infections all over the world. The aim of this study was to determine seroprevalence of HIV, HBV and HCV infections and high risk behaviors in persons who referred to the behavioral counseling center of Hamadan, west of Iran. This was a cross-sectional study which was done on 379 persons who referred to the behavioral counseling center of Hamadan. All persons after obtaining the informed consent were tested for serologic markers including HBs Ag, HCV-Ab and HIV-Ab by ELISA and western blot methods. Of the 379 persons, 71.5 % (271 cases) were male and 28.5% (108 cases) were female. HIV infection was reported in 4% (15) of persons. HBV and HCV infections were reported in 2.9% (11 cases) and 35.6% (135 cases), respectively. The most common high risk behaviors were injection drug user and history of prison with 52.5% (199 cases) and 40.4% (153 cases), respectively. According to the results, injection drug users and prisoners are at the highest risk for HCV, HIV and HBV infections.
    01/2011; 13(1):42-6.
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    ABSTRACT: Hepatitis B virus (HBV), the prototype member of the family hepadnaviridae, is a human pathogen, causing the serious liver disease. The virus is the major cause of chronic hepatitis cirrhosis, and hepatocellular carcinoma worldwide. HBV has been classified into eight genotypes, which vary in geographic distribution. The aim of this study was to recognize the HBV genotypes in hepatitis B surface antigen (HBsAg)-positive carriers in west of Iran. In this study, one hundred HBsAg-positive carriers were used. Genotypes of HBV were recognized by nested-PCR that combined by restriction fragment length polymorphism (RFLP) technique. Eighty six out of hundred samples were positive-PCR and were used for HBV genotyping. Recognized genotypes were D and B, with frequency of eighty five and one cases (out of eighty six samples) respectively, while no result was obtained in other fourteen samples. This study indicated that predominant genotype of HBV in west of Iran is genotype D, while genotype B is very rare in this region.
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