The in vitro Anti-Herpes Simplex Virus Type-1 and Type-2 Activity of Long Dan Xie Gan Tan, a Prescription of Traditional Chinese Medicine

Department of Cosmetic Applications and Management, Tung Fang Institute of Technology, Kaohsiung Medical University, Kaohsiung, Taiwan.
Chemotherapy (Impact Factor: 1.29). 02/2008; 54(2):77-83. DOI: 10.1159/000119705
Source: PubMed

ABSTRACT Long Dan Xie Gan Tan (LDXGT), a decoction of radix gentianae for purging the pathogenic inflammation of the liver, is a widely used prescription among many in traditional Chinese medicine. The prescription is primarily used to treat the disorders induced by damp-heat in the liver and the gall bladder.
In this study, the in vitro anti-herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) activity of the water extract of LDXGT was investigated.
LDXGT water extract was shown to exhibit anti-HSV activity. The IC(50) values of LDXGT against HSV-1 and HSV-2 infections were 257.5 +/- 12.2 and 494.6 +/- 1.8 microg/ml, respectively. It had a CC(50) value of 4,077.2 +/- 2.4 microg/ml towards Vero cells and showed no cytotoxic effect at a concentration of 2,000 microg/ml or below. The prescription was also found to inactivate HSV-2 infectivity in a dose-, time- and temperature-dependent manner.
In summary, the water extract of LDXGT was concluded to inhibit HSV-1 and HSV-2 infection at different magnitudes of potency, and our observations also suggested that the effect was likely mediated by directly inactivating the virus infectivity.

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    • "We have previously reported several natural products and herbal medicines that inhibit HSV infection and replication. For instance, ent-epiafzelechin-(4α→8)-epiafzelechin, extracted from Cassia javanica, inhibits HSV-2 replication; the herbal prescriptions Long-Dan-Xie-Gan-Tan (龍膽瀉肝湯 Lóng Dǎn Xiè Gān Tāng) and Yin-Chen-Hao-Tang (茵陳蒿湯 Yīn Chén Hāo Tang) both possess broad efficiency in diminishing HSV-1 and HSV-2 infectivity; hippomanin A, geraniin, 1,3,4,6-tetra-O-galloyl-beta-d-glucose, and excoecarianin isolated from Phyllanthus urinaria (葉下珠 Yè Xià Zhū) can potently impede HSV infection.[727374757677] In addition, we have also identified the hydrolyzable tannins chebulagic acid and punicalagin as cell surface glycosaminoglycan (GAG) competitors that can inhibit HSV-1 entry and cell-to-cell spread.[78] "
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