The in vitro Anti-Herpes Simplex Virus Type-1 and Type-2 Activity of Long Dan Xie Gan Tan, a Prescription of Traditional Chinese Medicine
ABSTRACT Long Dan Xie Gan Tan (LDXGT), a decoction of radix gentianae for purging the pathogenic inflammation of the liver, is a widely used prescription among many in traditional Chinese medicine. The prescription is primarily used to treat the disorders induced by damp-heat in the liver and the gall bladder.
In this study, the in vitro anti-herpes simplex virus type-1 (HSV-1) and type-2 (HSV-2) activity of the water extract of LDXGT was investigated.
LDXGT water extract was shown to exhibit anti-HSV activity. The IC(50) values of LDXGT against HSV-1 and HSV-2 infections were 257.5 +/- 12.2 and 494.6 +/- 1.8 microg/ml, respectively. It had a CC(50) value of 4,077.2 +/- 2.4 microg/ml towards Vero cells and showed no cytotoxic effect at a concentration of 2,000 microg/ml or below. The prescription was also found to inactivate HSV-2 infectivity in a dose-, time- and temperature-dependent manner.
In summary, the water extract of LDXGT was concluded to inhibit HSV-1 and HSV-2 infection at different magnitudes of potency, and our observations also suggested that the effect was likely mediated by directly inactivating the virus infectivity.
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- "We have previously reported several natural products and herbal medicines that inhibit HSV infection and replication. For instance, ent-epiafzelechin-(4α→8)-epiafzelechin, extracted from Cassia javanica, inhibits HSV-2 replication; the herbal prescriptions Long-Dan-Xie-Gan-Tan (龍膽瀉肝湯 Lóng Dǎn Xiè Gān Tāng) and Yin-Chen-Hao-Tang (茵陳蒿湯 Yīn Chén Hāo Tang) both possess broad efficiency in diminishing HSV-1 and HSV-2 infectivity; hippomanin A, geraniin, 1,3,4,6-tetra-O-galloyl-beta-d-glucose, and excoecarianin isolated from Phyllanthus urinaria (葉下珠 Yè Xià Zhū) can potently impede HSV infection. In addition, we have also identified the hydrolyzable tannins chebulagic acid and punicalagin as cell surface glycosaminoglycan (GAG) competitors that can inhibit HSV-1 entry and cell-to-cell spread. "
ABSTRACT: Viral infections play an important role in human diseases, and recent outbreaks in the advent of globalization and ease of travel have underscored their prevention as a critical issue in safeguarding public health. Despite the progress made in immunization and drug development, many viruses lack preventive vaccines and efficient antiviral therapies, which are often beset by the generation of viral escape mutants. Thus, identifying novel antiviral drugs is of critical importance and natural products are an excellent source for such discoveries. In this mini-review, we summarize the antiviral effects reported for several natural products and herbal medicines.03/2014; 4(1):24-35. DOI:10.4103/2225-4110.124335
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- "In fact, studies on TCM therapy for viral diseases are popular       . For the treatment of PRRS, many reports on TCM are available, especially when its clinical effect receives recognition from veterinary experts. "
ABSTRACT: To find an effective prevention method for porcine reproductive and respiratory syndrome (PRRS), three indices from experimental and control group pigs, namely, routine blood index, serum biochemical indica-tors, and conventional cell factor contents of serum, are inspected. The prevention and treatment of PRRS effect are evaluated, feeding pigs with Daitai. Results show that traditional Chinese medicine (TCM) Daitai can significantly improve the nonspecific immunity of pigs.
- Human Respiratory Syncytial Virus Infection, 11/2011; , ISBN: 978-953-307-718-5