Article

CYP450 biosensors based on gold chips for antiepileptic drugs determination.

Department of Analytical Chemistry, Faculty of Sciences, University of Burgos, Burgos, Spain.
Biosensors & Bioelectronics (Impact Factor: 6.45). 07/2008; 23(11):1733-7. DOI: 10.1016/j.bios.2008.01.030
Source: PubMed

ABSTRACT Three-electrode configuration chips containing a Pt, Au and a screen-printed Ag/AgCl as counter, working and reference electrode, respectively, have been developed. Selective determination of Phenobarbital (PB) has been carried out by Cytochrome P450 2B4 (CYP450) immobilization into a polypyrrole matrix onto the gold working electrode. Chronoamperometric experiments show a PB diffusion coefficient of 2.42x10(-6)cm(2)s(-1), a reproducibility and repeatability in terms of residual standard deviation (RSD) of 13% and 5.51%, respectively, and a limit of detection (LOD) of 0.289 micromol dm(-3) (alpha=beta=0.05) for the developed CYP450-biosensor chip. Its performance has been showed by the determination of PB in pharmaceutical drugs. HPLC has been used as reference technique.

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