Article

Plasmid mediated antibiotic resistance in marine bacteria

CAS in Marine Biology, Annamalai University, Parangipettai-608 502, India.
Journal of Environmental Biology (Impact Factor: 0.55). 08/2007; 28(3):617-21.
Source: PubMed

ABSTRACT This research work was conducted in Uppanar estuary to ascertain the role of plasmids in the antibiotic resistance of bacteria. Water and sediment samples were collected for a period of three months. When tested against 20 antibiotics 22 MAR strains were isolated from the samples, which were found resistant to 5-13 antibiotics. They belong to 7 genera and 10 species. Gram-negative bacteria namely Neisseria mucosa, N. sicca, Branhamella catarrhalis, Klebsiella ozaenae, Citrobacterintermedius, Pseudomonas fluorescens and Enterobacter aerogenes were isolated. Gram-positive bacteria were of Bacillus subtilis, B. megaterium and Micrococcus luteus. When plasmid curing was done using acredine orange, the resistance against penicillin-G, ampicillin, tetracycline, amoxycillin, kanamycin, and chloramphenicol were totally lost in all strains, which confirmed the role of plasmid in these strains against antibiotics. Ten strains belong to different species were selected for the plasmid isolation and electrophoresis was done. Presence of plasmids in all strains was confirmed and the molecular weight was in the range of 2850 to 3170 bp. The study revealed that MAR strains are common in Uppanar estuary and they are plasmid mediated. This environment is seemed to be deteriorating at an alarming rate.

Download full-text

Full-text

Available from: Thavasi Renga Thavasi, Aug 10, 2015
1 Follower
 · 
265 Views
  • Source
    • "Because of its small size and its already proven versatility as part of a shuttle plasmid, pMCE2 appears to be a rather promising candidate as a cloning device and gene vehicle. By acridine orange-mediated curing, Thavasi et al. (2007) reported that the loss of a plasmid of approximately 2.8 kb from a marine M. luteus was accompanied by the loss of penicillin, ampicillin, tetracycline, amoxicillin, kanamycin , and chloramphenicol resistance (Table 1). Similarly, a 5.1-kb plasmid from a Micrococcus strain isolated from poultry litter conferred multiple antibiotic resistance (kanamycin , tetracycline, erythromycin, ampicillin, tobramycin, streptomycin, rifampicin, and chloramphenicol) that was transferable. "
    Applied Microbiology and Biotechnology 01/2013; 97:63-75. · 3.81 Impact Factor
  • Source
    • "Because of its small size and its already proven versatility as part of a shuttle plasmid, pMCE2 appears to be a rather promising candidate as a cloning device and gene vehicle. By acridine orange-mediated curing, Thavasi et al. (2007) reported that the loss of a plasmid of approximately 2.8 kb from a marine M. luteus was accompanied by the loss of penicillin, ampicillin, tetracycline, amoxicillin, kanamycin , and chloramphenicol resistance (Table 1). Similarly, a 5.1-kb plasmid from a Micrococcus strain isolated from poultry litter conferred multiple antibiotic resistance (kanamycin , tetracycline, erythromycin, ampicillin, tobramycin, streptomycin, rifampicin, and chloramphenicol) that was transferable. "
    [Show abstract] [Hide abstract]
    ABSTRACT: Micrococci are Gram-positive G + C-rich, nonmotile, nonspore-forming actinomycetous bacteria. Micrococcus comprises ten members, with Micrococcus luteus being the type species. Representatives of the genus play important roles in the biodegradation of xenobiotics, bioremediation processes, production of biotechnologically important enzymes or bioactive compounds, as test strains in biological assays for lysozyme and antibiotics, and as infective agents in immunocompromised humans. The first description of plasmids dates back approximately 28 years, when several extrachromosomal elements ranging in size from 1.5 to 30.2 kb were found in Micrococcus luteus. Up to the present, a number of circular plasmids conferring antibiotic resistance, the ability to degrade aromatic compounds, and osmotolerance are known, as well as cryptic elements with unidentified functions. Here, we review the Micrococcus extrachromosomal traits reported thus far including phages and the only quite recently described large linear extrachromosomal genetic elements, termed linear plasmids, which range in size from 75 kb (pJD12) to 110 kb (pLMA1) and which confer putative advantageous capabilities, such as antibiotic or heavy metal resistances (inferred from sequence analyses and curing experiments). The role of the extrachromosomal elements for the frequently proven ecological and biotechnological versatility of the genus will be addressed as well as their potential for the development and use as genetic tools.
    Applied Microbiology and Biotechnology 11/2012; 97(1). DOI:10.1007/s00253-012-4539-5 · 3.81 Impact Factor
  • Source
    • "The number of antibiotics involved in the multiple drug resistance as obtained in this study, falls within the range obtained by earlier worker (Khan and Malik, 2001; Lateef, 2004; Paramasivam et al., 2007; Thavasi et al., 2007). Multiple drug-resistance is an extremely serious public health problem (Prescott et al., 1999). "
    [Show abstract] [Hide abstract]
    ABSTRACT: An experiment was designed to examine microbiology of water samples of Gölbasi lake and to assess the occurence of multiple antibiotic resistances in Escherichia coli from them. Total aerobic bacteria number in the lake was determined as 20x10(3) cfu ml(-1) and fecal coliforms were determined >1100 MPN/100 ml. Collected water samples from four geographically dispersed stations were screened for the E. coli and assessed for their resistance to twelve different antibiotics, which are commonly encountered in the lake. Of the total 13 E. coli isolates, 0% were susceptible to all antibiotics. All of isolates were found resistant to Penicillin (P) (100%). Among the twelve antibiotics tested, four patterns of antibiotic resistance were obtained and all of them were multiple antibiotic resistance with the number of antibiotics ranging from 2 to 5. Three isolates had beta-lactamase detected by iodometric slide test. The results indicated that persistent use of antibiotics against human diseases and other life forms may pollute the lake water and their impact on developing antibiotic resistant E. coli may be a serious threat in both health and environment.
    Journal of Environmental Biology 01/2009; 30(1):33-8. · 0.55 Impact Factor
Show more