Biorepositories - at the bleeding edge

International Journal of Epidemiology (Impact Factor: 9.2). 05/2008; 37(2):231-3. DOI: 10.1093/ije/dym282
Source: PubMed
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    ABSTRACT: Ngadze, E., Icishahayo, D., Coutinho, T. A., and van der Waals, J. E. 2012. Role of polyphenol oxidase, peroxidase, phenylalanine ammonia lyase, chlorogenic acid, and total soluble phenols in resistance of potatoes to soft rot. Plant Dis. 96:186-192. Pectobacterium atrosepticum, P carotovorum subsp. brasiliensis, and Dickeya spp. cause soft rot of potato (Solarium tuberosum) worldwide. Plants respond to bacterial invasion by activating defense responses associated with accumulation of several enzymes and inhibitors, which prevent pathogen infection. This study focused on the role of polyphenol oxidase (PPO), peroxidase (POD), phenylalanine ammonia lyase (PAL), chlorogenic acid, and total soluble phenols in imparting resistance to soft rot pathogens. Seven and II varieties grown by farmers in South Africa and Zimbabwe, respectively, were used in the study. The results showed significantly higher (P<0.001) enzyme activity of PPO and PAL as well as higher concentrations of chlorogenic acid and total soluble phenols in Vanderplank, Pentland Dell, M69/11, Romano, M59/20, and Mondial(Zw). PAL activity increased significantly with time in all varieties, and the highest activity was recorded 8 h after cutting. The resistance of the varieties was correlated with high PPO and PAL enzyme activity as well as increased concentrations of chlorogenic acid and total soluble phenols. PPO, POD, and PAL activities increased significantly in wounded and inoculated tubers. These findings show that PAL, PPO, POD, chlorogenic acid, and total soluble phenols play a role in imparting resistance to potato soft rot infection.
    Plant Disease 02/2012; 96(2):186-192. DOI:10.1094/PDIS-02-11-0149 · 2.74 Impact Factor
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    ABSTRACT: A global interest in therapies for neglected diseases is rising, but traditional biopharma research and development (R&D) process is prohibitively expensive to justify cost of their development. Vitiligo is a multifactorial orphan disease that affects at minimum 35 million people worldwide, yet no therapeutic solutions exist. The present authors describe a budget-minded pursuit of the new therapy development for vitiligo, which includes a multidiscipline collaboration and effective bridging between academic research, biobanking, and bioinformatics. The present authors anticipate that the present authors' "theoretically induced and empirically guided" discovery process will enable development of more leads, with a much greater probability of success and under tighter budgets compared with those of the biopharma company. Ultimately, the multidisciplinary approach described below facilitates the collaborative development of personalized treatments for different patient subpopulations in vitiligo and other neglected diseases.
    Dermatologic Therapy 11/2012; 25 Suppl 1:S1-9. DOI:10.1111/dth.12009 · 1.48 Impact Factor
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    ABSTRACT: Today's biobanks must work to take full advantage of collected samples, while maximizing sample quality and minimizing costs to sustain operations for a long period of time. This is a tall order that will require collaboration and compromise for both end-users and collection sites. This article discusses the efforts of the Génome Québec-Centre Hospitalier Affilié Universitaire Régional de Chicoutimi Biobank to fractionate blood samples for the simultaneous preservation of plasma and DNA-containing layers while minimizing resources required for shipping and transport. This article also describes methods for successful reproducible application of the plasma-depleted blood sample to GenPlates (GenVault, Carlsbad, CA).
    Biopreservation and Biobanking 12/2010; 8(4):193-6. DOI:10.1089/bio.2010.0017 · 1.58 Impact Factor